J1020
1020 .
DESULFOCARBO INDIANENSIS MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1020 #
ml
Equipment needed: Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2SO4 | 2.000 | g | 1.843 | 12.978 | |
| NaCl | 8.800 | g | 8.111 | 138.785 | |
| MgCl2 x 6 H2O | 4.600 | g | 4.24 | 20.854 | |
| KCl | 0.340 | g | 0.313 | 4.203 | |
| NH4Cl | 0.250 | g | 0.23 | 4.308 | |
| KH2PO4 | 0.140 | g | 0.129 | 0.948 | |
| CaCl2 x 2 H2O | 0.140 | g | 0.129 | 0.878 | |
| NiCl2 x 6 H2O | 9.300 | mg | 0.009 | 0.066 | |
| Fe(NH4)2(SO4)2 x 6 H2O | 2.700 | mg | 0.002 | - | |
| Na2SeO3 x 5 H2O | 0.260 | mg | 2.4e-4 | 9.1e-4 | |
| Na2WO4 x 2 H2O | 0.084 | mg | 7.7e-5 | 2.3e-4 | |
| Trace element solution | 10.000 | ml | - | - | |
| Trace vitamins | 10.000 | ml | - | - | |
| Sodium benzoate | 0.360 | g | 0.332 | 2.303 | |
| Resazurin | 1.000 | mg | 9.2e-4 | 0.004 | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Mix components thoroughly and distribute the medium into culture vessels (e.g., 10 ml medium in Balch-type tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions: | |||||
|
NaHCO3
(filter-sterilized, 8%) |
60.000 | ml | - | - | |
|
Na2S x 9 H2O
(autoclaved, 5%) |
5.000 | ml | - | - | |
Solution occurs in 89
other media (first defined in Medium J187).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| ZnCl2 | 70 | mg | 0.07 | 0.514 |
| MnCl2 x 4 H2O | 100 | mg | 0.1 | 0.505 |
| H3BO3 | 6 | mg | 0.006 | 0.097 |
| CoCl2 x 6 H2O | 190 | mg | 0.19 | 1.463 |
| CuCl2 x 2 H2O | 2 | mg | 0.002 | 0.012 |
| NiCl2 x 6 H2O | 24 | mg | 0.024 | 0.185 |
| Na2MoO4 x 2 H2O | 36 | mg | 0.036 | 0.149 |
| Distilled water | 1000 | ml | - | - |
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 29.10.21 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | n.d. |
| Equipment needed: | Anaerobic workbench, Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 8.111 | 138.785 |
| MgCl2 x 6 H2O | 4.24 | 20.854 |
| Na2SO4 | 1.843 | 12.978 |
| Sodium benzoate | 0.332 | 2.303 |
| KCl | 0.313 | 4.203 |
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