J1023
1023 .
PYVL MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1023 #
ml
Equipment needed: Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Salt Solution I
(see Medium No. 133) |
75.0 | ml | - | - | |
|
Salt Solution II
(see Medium No. 133) |
75.0 | ml | - | - | |
|
Trypticase peptone
(BD-BBL) |
10.0 | g | 9.96 | - | |
|
Yeast extract
(BD-Difco) |
5.0 | g | 4.98 | - | |
| L-Lysine | 5.0 | g | 4.98 | 34.066 | |
| Vitamin mixture | 1.0 | ml | - | - | |
|
Sodium resazurin
(w/v, 0.1%) |
1.0 | ml | - | - | |
|
Na2CO3
(w/v, 8%) |
2.5 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.3 | g | 0.299 | - | |
| Distilled water | 850.0 | ml | - | - | |
| 1 Adjust pH to 7.3. Prepare medium anaerobically and pour into rubber stopped tubes under oxygen-free N2-CO2 (95:5). | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 1 | mg | 0.01 | 0.041 |
| Vitamin B12 | 1 | mg | 0.01 | 0.007 |
| p-Aminobenzoic acid | 3 | mg | 0.03 | 0.219 |
| Folic acid | 5 | mg | 0.05 | 0.113 |
| Thiamine HCl | 5 | mg | 0.05 | - |
| Riboflavin | 5 | mg | 0.05 | 0.133 |
| Pyridoxamine hydrochloride | 15 | mg | 0.15 | - |
| Distilled water | 100 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.3 |
| Equipment needed: | Anaerobic workbench |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Trypticase peptone | 9.96 | - |
| L-Lysine | 4.98 | 34.066 |
| Yeast extract | 4.98 | - |
| L-Cysteine HCl x H2O | 0.299 | - |
| Pyridoxamine hydrochloride | 1.5e-4 | - |
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