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JCM 1024 . THERMUS 162 MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1024 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract
(BD-Difco) 		1.00 g 0.999 -
Tryptone
(BD-Difco) 		1.00 g 0.999 14.055
Nitrilotriacetic acid 0.10 g 0.1 0.523
CaSO4 x 2 H2O 0.04 g 0.04 0.232
MgCl2 x 6 H2O 0.20 g 0.2 0.983
Iron(III) citrate
(0.01 M) 		0.50 ml - -
Nitsch's trace elements 0.50 ml - -
Distilled water 900.00 ml - -
1 Adjust pH to 7.2 with NaOH. For preparation of solid medium, add 28.0 g/L agar. After autoclaving, add the following solution to the medium:
Phosphate buffer 100.00 ml - -

Phosphate buffer #

1000
Solution occurs in 3 other media (first defined in Medium 630).
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 5.44 g 5.44 39.975
Na2HPO4 x 12 H2O 43.00 g 43 302.904
Distilled water 1000.00 ml - -
1 Adjust pH to 7.2

Nitsch's trace elements #

1000
Solution occurs in 1 other media (first defined in Medium J273).
Compound Amount Unit Conc. [g/L] Conc. [mM]
H2SO4 0.500 ml - -
MnSO4 x n H2O 2.200 g 2.2 -
ZnSO4 x 7 H2O 0.500 g 0.5 1.739
H3BO3 0.500 g 0.5 8.085
CuSO4 x 5 H2O 0.016 g 0.016 0.064
Na2MoO4 x 2 H2O 0.025 g 0.025 0.103
CoCl2 x 6 H2O 0.046 g 0.046 0.354
Distilled water 1000.000 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
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Associated strains

All strains for this medium: 5

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 x 12 H2O 4.296 30.26
Tryptone 0.999 14.055
Yeast extract 0.999 -
KH2PO4 0.543 3.993
MgCl2 x 6 H2O 0.2 0.983
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Cultivation metadata from 5 strains BacDive

pH 4 - 6 2 strains
pH 6 - 8 2 strains
pH 8 - 10 1 strain
pH 10 - 12 1 strain
phyla 2 5 strains
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