Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Yeast extract
(BD-Difco) |
1.00 | g | ||
|
Tryptone
(BD-Difco) |
1.00 | g | ||
|
Nitrilotriacetic acid | 0.10 | g | ||
|
CaSO4 x 2 H2O | 0.04 | g | ||
|
MgCl2 x 6 H2O | 0.20 | g | ||
|
Iron(III) citrate
(0.01 M) |
0.50 | ml | ||
|
Nitsch's trace elements | 0.50 | ml | ||
|
Distilled water | 900.00 | ml | ||
1 Adjust pH to 7.2 with NaOH. For preparation of solid medium, add 28.0 g/L agar. After autoclaving, add the following solution to the medium: | |||||
|
Phosphate buffer | 100.00 | ml |
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
KH2PO4 | 5.44 | g | ||
|
Na2HPO4 x 12 H2O | 43.00 | g | ||
|
Distilled water | 1000.00 | ml | ||
1 Adjust pH to 7.2 |
Compound | Amount | Unit | |
---|---|---|---|
|
H2SO4 | 0.500 | ml |
|
MnSO4 x n H2O | 2.200 | g |
|
ZnSO4 x 7 H2O | 0.500 | g |
|
H3BO3 | 0.500 | g |
|
CuSO4 x 5 H2O | 0.016 | g |
|
Na2MoO4 x 2 H2O | 0.025 | g |
|
CoCl2 x 6 H2O | 0.046 | g |
|
Distilled water | 1000.000 | ml |