J1025
1025 .
PYX MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1025 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Trypticase peptone | 5.0 | g | 4.808 | - | |
|
Bacto peptone
(BD-Difco) |
5.0 | g | 4.808 | - | |
|
Yeast extract
(BD-Difco) |
10.0 | g | 9.615 | - | |
| Glucose | 2.0 | g | 1.923 | 10.674 | |
| Resazurin | 1.0 | mg | 9.6e-4 | 0.004 | |
| Salt solution | 40.0 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.481 | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients (except glucose and cysteine), boil medium for 1 min, then cool to room temperature under a N2 gas atmosphere. Add cysteine and adjust pH to 7.0. Thereafter, dispense into culture vessels under a N2 gas atmosphere and autoclave. After autoclaving, add glucose from a sterile, anoxic stock solution prepared under a N2 gas atmosphere. | |||||
Solution occurs in 9
other media (first defined in Medium 104).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| CaCl2 x 2 H2O | 0.25 | g | 0.25 | 1.701 |
| MgSO4 x 7 H2O | 0.50 | g | 0.5 | 2.029 |
| K2HPO4 | 1.00 | g | 1 | 5.741 |
| KH2PO4 | 1.00 | g | 1 | 7.348 |
| NaHCO3 | 10.00 | g | 10 | 119.038 |
| NaCl | 2.00 | g | 2 | 34.223 |
| Distilled water | 1000.00 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Yeast extract | 9.615 | - |
| Trypticase peptone | 4.808 | - |
| Bacto peptone | 4.808 | - |
| Glucose | 1.923 | 10.674 |
| L-Cysteine HCl x H2O | 0.481 | - |
| Show more | ||
