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JCM 1026 . IRD MARINIFILUM MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.0 g 0.999 18.676
KH2PO4 0.3 g 0.3 2.202
K2HPO4 0.3 g 0.3 1.721
NaCl 25.0 g 24.975 427.362
KCl 0.1 g 0.1 1.34
CaCl2 x 2 H2O 0.1 g 0.1 0.68
Yeast extract 1.0 g 0.999 -
Trace element solution 1.0 ml - -
L-Cysteine HCl x H2O 0.5 g 0.5 -
Resazurin 1.0 mg 1.0e-3 0.004
Distilled water 925.0 ml - -
1 Mix components thoroughly and adjust pH to 7.2. Bring to a boil, cool down under a N2-CO2 (4:1, v/v) gas stream, seal with butyl rubber stoppers and autoclave. Aseptically and anaerobically add the following stock solutions [autoclaved and stored under a N2 atmosphere or *filter-sterilize and store under a N2-CO2 (4:1, v/v) gas mixture]:
MgCl2 x 6 H2O
(15%)
20.0 ml - -
NaHCO3
(*, 8%)
25.0 ml - -
Glucose
(1 M)
20.0 ml - -
Na2S x 9 H2O
(5%)
10.0 ml - -
2 Readjust pH to 7.2, if necessary.
Solution occurs in 38 other media (first defined in Medium J439).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
12.5 ml - -
FeSO4 x 7 H2O 2.1 g 2.1 7.554
H3BO3 30.0 mg 0.03 0.485
MnCl2 x 4 H2O 100.0 mg 0.1 -
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
ZnSO4 x 7 H2O 144.0 mg 0.144 0.501
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 987.0 ml - -

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Anaerobic workbench, Autoclave, Filter

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 24.975 427.362
NH4Cl 0.999 18.676
Yeast extract 0.999 -
L-Cysteine HCl x H2O 0.5 -
KH2PO4 0.3 2.202
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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