Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NH4Cl | 0.54 | g | 0.523 | 9.773 | |
KH2PO4 | 0.14 | g | 0.136 | 0.996 | |
MgCl2 x 6 H2O | 3.50 | g | 3.388 | 16.666 | |
K2HPO4 | 0.30 | g | 0.29 | 1.667 | |
CaCl2 x 2 H2O | 0.15 | g | 0.145 | 0.988 | |
NaCl | 5.00 | g | 4.84 | 82.825 | |
NaHCO3 | 2.52 | g | 2.44 | 29.039 | |
Trace mineral solution | 1.00 | ml | - | - | |
Se/W solution | 1.00 | ml | - | - | |
Trace vitamins solution | 1.00 | ml | - | - | |
Resazurin | 1.00 | mg | 9.7e-4 | 0.004 | |
Distilled water | 1000.00 | ml | - | - | |
1 Mix components thoroughly and distribute the medium into culture vessels (e.g., 20 ml medium in 100 ml serum bottles or 5.0 ml medium in Hungate tubes) under a stream of H2-CO2 (80:20, v/v), seal with butyl rubber stoppers and autoclave. Allow to stand the autoclaved medium overnight. Add per liter the following solution from a sterile anaerobic stock (filter-sterilized and stored under a N2 atmosphere): | |||||
Trimethylamine
(1 M) |
10.00 | ml | - | - | |
2 Prior to inoculation, add per liter the following solutions aseptically and anaerobically (autoclaved and stored under a N2 atmosphere): | |||||
L-Cysteine HCl x H2O
(5%) |
10.00 | ml | - | - | |
Na2S x 9 H2O
(5%) |
10.00 | ml | - | - | |
3 Comments: For strain JCM 19935, replace trimethylamine with yeast extract (final 0.1 g/L) and pressurize to 100 kPa H2-CO2 (80:20, v/v). For strain JCM 19936, prepare the medium without trimethylamine. Pressurize inoculated culture vessels to 100 kPa H2-CO2 (80:20, v/v). |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
FeCl2 x 4 H2O | 1.990 | g | 1.99 | 10.01 |
MnCl2 x 4 H2O | 0.198 | g | 0.198 | - |
CoCl2 x 6 H2O | 0.130 | g | 0.13 | 1.001 |
ZnCl2 | 0.136 | g | 0.136 | 0.998 |
CuCl2 x 2 H2O | 1.700 | mg | 0.002 | 0.01 |
H3BO3 | 6.200 | mg | 0.006 | 0.1 |
Na2MoO4 x 2 H2O | 0.024 | g | 0.024 | 0.099 |
NiCl2 x 6 H2O | 0.013 | g | 0.013 | 0.1 |
AlCl3 | 0.013 | g | 0.013 | 0.097 |
Distilled water | 1000.000 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Na2SeO3 x 5 H2O | 1.7 | mg | 0.002 | 0.006 |
Na2WO4 x 2 H2O | 3.3 | mg | 0.003 | 0.01 |
Distilled water | 1000.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 4.9 | mg | 0.005 | 0.02 |
Folic acid | 8.8 | mg | 0.009 | 0.02 |
Pyridoxine hydrochloride | 4.1 | mg | 0.004 | - |
Thiamine HCl | 6.7 | mg | 0.007 | - |
Riboflavin | 7.5 | mg | 0.008 | 0.02 |
Nicotinic acid | 2.5 | mg | 0.003 | 0.02 |
DL-Calcium pantothenate | 9.5 | mg | 0.01 | 0.02 |
Vitamin B12 | 27.1 | mg | 0.027 | 0.02 |
p-Aminobenzoic acid | 2.7 | mg | 0.003 | 0.02 |
Lipoic acid | 4.1 | mg | 0.004 | 0.02 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 29.10.21 |
Source: | JCM |
Medium type: | Defined medium |
Final pH: | n.d. |
Equipment needed: | Anaerobic workbench, Autoclave, Filter, Hungate tubes |
Edit in Medium builder |