Name: IRD SM40 MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J1034 #

Equipment needed: Filter Autoclave Anaerobic workbench

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Na₂SO₄	3.16	g	3.132	22.049
NH₄Cl	1.00	g	0.991	18.528
KH₂PO₄	0.30	g	0.297	2.185
K₂HPO₄	0.30	g	0.297	1.707
NaCl	40.00	g	39.643	678.357
KCl	0.20	g	0.198	2.659
CaCl₂ x 2 H₂O	0.10	g	0.099	0.674
Sodium acetate	0.50	g	0.496	6.041
Sodium lactate	6.00	g	5.946	53.065
Yeast extract	0.50	g	0.496	-
Trace element solution	1.00	ml	-	-
Resazurin	1.00	mg	9.9e-4	0.004
Distilled water	955.00	ml	-	-
1 Mix components thoroughly and adjust pH to 7.5 with KOH. Bring to a boil, cool down under a N₂-CO₂ (4:1, v/v) gas stream, seal with butyl rubber stoppers and autoclave. Aseptically and anaerobically add the following stock solutions [autoclaved and stored under a N₂ atmosphere or *filter-sterilize and store under a N₂-CO₂ (4:1, v/v) gas mixture]:
MgSO₄ x 7 H₂O (15%)	20.00	ml	-	-
NaHCO₃ (*, 8%)	25.00	ml	-	-
Na₂S x 9 H₂O (5%)	8.00	ml	-	-
2 Readjust pH to 7.5, if necessary.

Trace element solution #

1000

Solution occurs in 38 other media (first defined in Medium J439).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
HCl (25%)	12.5	ml	-	-
FeSO₄ x 7 H₂O	2.1	g	2.1	7.554
H₃BO₃	30.0	mg	0.03	0.485
MnCl₂ x 4 H₂O	100.0	mg	0.1	-
CoCl₂ x 6 H₂O	190.0	mg	0.19	1.463
NiCl₂ x 6 H₂O	24.0	mg	0.024	0.185
CuCl₂ x 2 H₂O	2.0	mg	0.002	0.012
ZnSO₄ x 7 H₂O	144.0	mg	0.144	0.501
Na₂MoO₄ x 2 H₂O	36.0	mg	0.036	0.149
Distilled water	987.0	ml	-	-

Last modified:	22.02.22
Source:	JCM
Taxonomic range:	Bacteria
Medium type:	Complex medium
Final pH:	7.5
Equipment needed:	Anaerobic workbench, Autoclave, Filter

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phyla	1	1 strain
temperature	mesophilic	1 strain