J1041
1041 .
THIOBACILLUS PROSPERUS FO1 MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1041 #
ml
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KCl | 0.33 | g | 0.33 | 4.427 | |
| MgCl2 x 6 H2O | 2.75 | g | 2.75 | 13.527 | |
| NH4Cl | 1.25 | g | 1.25 | 23.369 | |
| CaCl2 x 2 H2O | 0.14 | g | 0.14 | 0.952 | |
| KH2PO4 | 0.14 | g | 0.14 | 1.029 | |
| K2HPO4 | 0.14 | g | 0.14 | 0.804 | |
| NaCl | 20.00 | g | 20 | 342.231 | |
| Distilled water | 940.00 | ml | - | - | |
| 1 Adjust pH to 6.0 and autoclave. After cooling, aseptically add the following solutions (filter-sterilized): | |||||
| Trace mineral solution | 10.00 | ml | - | - | |
| FeSO4 x 7H2O solution | 50.00 | ml | - | - | |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Trace minerals | 1000.000 | ml | - | - |
| NiCl2 x 6 H2O | 0.225 | g | 0.225 | 1.736 |
| Na2SeO3 x 5 H2O | 0.300 | g | 0.3 | 1.141 |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| FeSO4 x 7 H2O | 2 | g | 20 | 71.939 | |
|
H2SO4
(0.1 N) |
100 | ml | - | - | |
| 1 Prepare just before use. | |||||
Solution occurs in 97
other media (first defined in Medium J151).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
| MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
| MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
| CoSO4 x 7 H2O | 0.10 | g | 0.1 | 0.645 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
| CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
| AlK(SO4)2 | 0.01 | g | 0.01 | 0.039 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Defined medium |
| Final pH: | 6.0 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |