J1042
1042 .
CALROANAEROBACTER MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1042 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Yeast extract
(BD-Difco) |
2.00 | g | 1.99 | - | |
|
Tryptone
(BD-Difco) |
2.00 | g | 1.99 | 27.997 | |
| Sodium pyruvate | 5.00 | g | 4.975 | 45.212 | |
| PIPES | 3.46 | g | 3.443 | 11.385 | |
| Trace vitamins | 5.00 | ml | - | - | |
| Artificial seawater | 1000.00 | ml | - | - | |
| 1 Adjust pH to 7.0. Distribute the medium into culture vessels under a N2 gas atmosphere, seal with butyl rubber stoppers and autoclave. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaCl | 22.00 | g | 21.891 | 374.582 |
| MgCl2 x 6 H2O | 3.00 | g | 2.985 | 14.683 |
| MgSO4 x 7 H2O | 6.00 | g | 5.97 | 24.222 |
| (NH4)2SO4 | 1.00 | g | 0.995 | 7.53 |
| NaHCO3 | 0.20 | g | 0.199 | 2.369 |
| CaCl2 x 2 H2O | 0.30 | g | 0.299 | 2.03 |
| KCl | 0.50 | g | 0.498 | 6.674 |
| KH2PO4 | 0.42 | g | 0.418 | 3.071 |
| NaBr | 0.05 | g | 0.05 | 0.484 |
| SrCl2 x 6 H2O | 0.02 | g | 0.02 | 0.075 |
| Ammonium ferric citrate | 0.01 | g | 0.01 | - |
| Trace minerals | 5.00 | ml | - | - |
| Distilled water | 1000.00 | ml | - | - |
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Solution occurs in 97
other media (first defined in Medium J151).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
| MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
| MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
| CoSO4 x 7 H2O | 0.10 | g | 0.1 | 0.645 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
| CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
| AlK(SO4)2 | 0.01 | g | 0.01 | 0.039 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |