Name: IRD MARINE DESULFOVIBRIO MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J1043 #

Equipment needed: Anaerobic workbench Filter Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NH₄Cl	1.0	g	0.991	18.528
KH₂PO₄	0.3	g	0.297	2.185
K₂HPO₄	0.3	g	0.297	1.707
NaCl	25.0	g	24.777	423.973
KCl	0.1	g	0.099	1.329
CaCl₂ x 2 H₂O	0.1	g	0.099	0.674
Na₂SO₄	4.0	g	3.964	27.91
Yeast extract (BD-Difco)	1.0	g	0.991	-
Trace element solution	1.0	ml	-	-
L-Cysteine HCl x H₂O	0.5	g	0.496	-
Resazurin	1.0	mg	9.9e-4	0.004
Distilled water	920.0	ml	-	-
1 Mix ingredients and adjust pH to 7.0. Bring to a boil and cool down under a N₂-CO₂ (4:1, v/v) gas stream. Distribute the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized):
Sodium lactate (1 M)	20.0	ml	-	-
MgCl₂ x 6 H₂O (15%)	20.0	ml	-	-
NaHCO₃ (*, 5%)	40.0	ml	-	-
2 Prior to use, reduce the medium by the addition of the following solution (autoclaved and stored under N₂, per liter):
Na₂S x 9 H₂O (5%)	8.0	ml	-	-

Trace element solution #

1000

Solution occurs in 38 other media (first defined in Medium J439).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
HCl (25%)	12.5	ml	-	-
FeSO₄ x 7 H₂O	2.1	g	2.1	7.554
H₃BO₃	30.0	mg	0.03	0.485
MnCl₂ x 4 H₂O	100.0	mg	0.1	-
CoCl₂ x 6 H₂O	190.0	mg	0.19	1.463
NiCl₂ x 6 H₂O	24.0	mg	0.024	0.185
CuCl₂ x 2 H₂O	2.0	mg	0.002	0.012
ZnSO₄ x 7 H₂O	144.0	mg	0.144	0.501
Na₂MoO₄ x 2 H₂O	36.0	mg	0.036	0.149
Distilled water	987.0	ml	-	-

Last modified:	22.02.22
Source:	JCM
Taxonomic range:	Bacteria
Medium type:	Complex medium
Final pH:	7.0
Equipment needed:	Anaerobic workbench, Autoclave, Filter

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