Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 1.0 | g | 0.996 | 17.043 | |
1 Adjust pH to 7.1 - 7.2. Distribute the medium into culture vessels under the same gas mixture and seal with butyl rubber stoppers. Finally, add per liter the following solution (filter-sterilized): | |||||
KCl | 0.5 | g | 0.498 | 6.68 | |
MgCl2 x 6 H2O | 0.4 | g | 0.398 | 1.96 | |
CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.677 | |
NH4Cl | 0.3 | g | 0.299 | 5.586 | |
KH2PO4 | 0.2 | g | 0.199 | 1.464 | |
Resazurin | 0.4 | mg | 4.0e-4 | 0.002 | |
Distilled water | 945.0 | ml | - | - | |
2 Mix components thoroughly and autoclave. Cool medium under a N2-CO2 (4:1, v/v) gas stream, and add the following solutions (autoclaved or *filter-sterilized) anaerobically and aseptically: | |||||
NaHCO3
(*, 8%) |
30.0 | ml | - | - | |
Glucose
(*, 1 M) |
5.0 | ml | - | - | |
L-Cysteine HCl x H2O
(0.1 M) |
10.0 | ml | - | - | |
Yeast extract
(*, 10%) |
2.0 | ml | - | - | |
Casamino acids
(*, 10%) |
2.0 | ml | - | - | |
Trace element solution | 1.0 | ml | - | - | |
FeCl2 solution | 1.0 | ml | - | - | |
Selenite-tungstate solution | 1.0 | ml | - | - | |
Trace vitamins solution (see Medium No. 284)
(*) |
1.0 | ml | - | - | |
Fatty acid mixture | 1.0 | ml | - | - | |
Aromatic fatty acids solution | 1.0 | ml | - | - | |
Dithiothreitol
(250 mM) |
4.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Phenyl acetic acid | 68 | mg | 0.68 | 4.994 | |
Phenyl propionic acid | 75 | mg | 0.75 | 4.994 | |
4-Hydroxyphenyl acetic acid | 76 | mg | 0.76 | 4.995 | |
3-Indolyl acetic acid | 88 | mg | 0.88 | 5.023 | |
NaOH
(20 mM) |
100 | ml | - | - | |
1 Autoclave and store under a N2 atmosphere. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
ZnCl2 | 70 | mg | 0.07 | 0.514 |
MnCl2 x 4 H2O | 100 | mg | 0.1 | 0.505 |
H3BO3 | 6 | mg | 0.006 | 0.097 |
CoCl2 x 6 H2O | 190 | mg | 0.19 | 1.463 |
CuCl2 x 2 H2O | 2 | mg | 0.002 | 0.012 |
NiCl2 x 6 H2O | 24 | mg | 0.024 | 0.185 |
Na2MoO4 x 2 H2O | 36 | mg | 0.036 | 0.149 |
Distilled water | 1000 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
HCl
(7.7 M, 25%) |
10.0 | ml | 2.5 | 68.568 |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 |
Distilled water | 990.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaOH | 0.4 | g | 0.4 | 10.001 |
Na2SeO3 x 5 H2O | 6.0 | mg | 0.006 | 0.023 |
Na2WO4 x 2 H2O | 8.0 | mg | 0.008 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Valeric acid | 0.5 | g | 25 | 244.786 | |
Isovaleric acid | 0.5 | g | 25 | 244.786 | |
alpha-Methylbutyric acid | 0.5 | g | 25 | 244.786 | |
Isobutyric acid | 0.5 | g | 25 | 283.736 | |
Distilled water | 20.0 | ml | - | - | |
1 Adjust pH to 7.5 with concentrated NaOH solution. |
Last modified: | 22.02.22 |
Source: | JCM |
Medium type: | Complex medium |
Final pH: | 7.2 |
Equipment needed: | Anaerobic workbench, Autoclave, Filter |
Edit in Medium builder |