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J1053. DEFERRISOMA PALEOCHRORII MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 15.00 g 14.97 256.15
2 4.00 g 3.992 19.636
3 0.33 g 0.329 4.418
4 0.33 g 0.329 2.24
5 0.50 g 0.499 3.776
6 FeCl2 solution 1.00 ml - -
7 Trace element solution 1.00 ml - -
8 Selenite-tungstate solution 0.50 ml - -
9 860.00 ml 856.745 47556.5
10
(3.3%)
10.00 ml 1.515 11.133
11 Trace vitamins
(*)
10.00 ml - -
12
(*, 8%)
10.00 ml 1.724 20.519
13 This solution has been removed!
(w/v) MES (pH 6.0, 20%)
10.00 ml - -
14 Iron(III) citrate solution 100.00 ml - -
Instructions:
  1. Mix components thoroughly, adjust pH to 6.0 with 1 N HCl and autoclave under a H2-CO2 (4:1, v/v) gas mixture. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized): #10-14
  2. Readjust pH to 6.0 to 6.3, if necessary.
Solution occurs in 89 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 70 mg 0.07 0.514
2 100 mg 0.1 -
3 6 mg 0.006 0.097
4 190 mg 0.19 -
5 2 mg 0.002 -
6 24 mg 0.024 -
7 36 mg 0.036 0.149
8 1000 ml 998.207 55408.8
Solution occurs in 87 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(7.7 M, 25%)
10.0 ml 3.17 86.943
2 1.5 g 1.5 -
3 990.0 ml 988.225 54854.8
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 69 other media (first defined in medium J431 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.4 g 0.4 10.001
2 6.0 mg 0.006 -
3 8.0 mg 0.008 -
4 1000.0 ml 998.207 55408.8
Solution occurs in 2 other media (first defined in medium J1014 )
Instructions:
  1. Mix 2.4 g iron(III) citrate and 90 ml distilled water, and boil until dissolved. Cool to an ambient temperature and adjust pH to 6.0 with 10 N NaOH solution. Fill up to 100 ml with distilled water.

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 869.188 48247.2
NaCl 14.97 256.15
MgCl2 x 6H2O 3.992 19.636
NaHCO3 1.724 20.519
KH2PO4 1.515 11.133
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