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JCM 1053 . DEFERRISOMA PALEOCHRORII MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1053 #

ml
Equipment needed: Anaerobic workbench Filter Autoclave
1 Mix components thoroughly, adjust pH to 6.0 with 1 N HCl and autoclave under a H2-CO2 (4:1, v/v) gas mixture. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 15.00 g 14.97 256.161
MgCl2 x 6 H2O 4.00 g 3.992 19.636
KCl 0.33 g 0.329 4.418
CaCl2 x 2 H2O 0.33 g 0.329 2.24
(NH4)2SO4 0.50 g 0.499 3.776
FeCl2 solution 1.00 ml - -
Trace element solution 1.00 ml - -
Selenite-tungstate solution 0.50 ml - -
Distilled water 860.00 ml - -
KH2PO4
(3.3%) 		10.00 ml - -
Trace vitamins
(*) 		10.00 ml - -
NaHCO3
(*, 8%) 		10.00 ml - -

(w/v) MES (pH 6.0, 20%) 		10.00 ml - -
Iron(III) citrate solution 100.00 ml - -
2 Readjust pH to 6.0 to 6.3, if necessary.

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Selenite-tungstate solution #

1000
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Iron(III) citrate solution #

Solution occurs in 2 other media (first defined in Medium J1014).
1 Mix 2.4 g iron(III) citrate and 90 ml distilled water, and boil until dissolved. Cool to an ambient temperature and adjust pH to 6.0 with 10 N NaOH solution. Fill up to 100 ml with distilled water.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.0
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 14.97 256.161
MgCl2 x 6 H2O 3.992 19.636
(NH4)2SO4 0.499 3.776
KCl 0.329 4.418
CaCl2 x 2 H2O 0.329 2.24
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
phyla 1 1 strain
nutrition mixotroph 1 strain
oxygen obligate anaerobe 1 strain
temperature thermophilic 1 strain