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JCM 1062 . DESULFUROMONAS CARBONIS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1062 #

ml
Equipment needed: Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 8.800 g 8.148 139.428
MgCl2 x 6 H2O 4.600 g 4.259 20.95
KCl 0.340 g 0.315 4.223
NH4Cl 0.250 g 0.231 4.328
KH2PO4 0.140 g 0.13 0.953
CaCl2 x 2 H2O 0.140 g 0.13 0.882
NiCl2 x 6 H2O 9.300 mg 0.009 0.066
Fe(NH4)2(SO4)2 x 6 H2O 2.700 mg 0.003 -
Na2SeO3 x 5 H2O 0.260 mg 2.4e-4 9.2e-4
Na2WO4 x 2 H2O 0.084 mg 7.8e-5 2.4e-4
Trace element solution 10.000 ml - -
Trace vitamins 10.000 ml - -
Disodium fumarate 8.000 g 7.407 46.285
Distilled water 1000.000 ml - -
1 Mix components thoroughly, distribute the medium into culture vessels (e.g., 10 ml medium in Balch-type tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions:
NaHCO3
(filter-sterilized, 8%) 		60.000 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Anaerobic workbench, Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 8.148 139.428
Disodium fumarate 7.407 46.285
MgCl2 x 6 H2O 4.259 20.95
KCl 0.315 4.223
NH4Cl 0.231 4.328
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain