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J1064. GEOSPOROBACTER MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.50 g 0.499 6.693
2 0.20 g 0.2 1.467
3 1.00 g 0.998 17.077
4 0.05 g 0.05 0.933
5 0.10 g 0.1 0.679
6 0.40 g 0.399 1.964
7 1.00 g 0.998 -
8 0.36 g 0.359 1.994
9 Trace vitamins 1.00 ml - -
10 Trace element solution 1.00 ml - -
11 0.84 g 0.838 9.979
12 0.50 g 0.499 2.078
13 1.00 mg 1.0e-3 0.004
14 1000.00 ml 996.215 55298.2
Instructions:
  1. Mix ingredients except NaHCO3, Trace vitamins and Na2S x 9H2O, and autoclave under a N2-CO2 (90:10) atmosphere. Filter-sterilize NaHCO3 as an 8.4% solution and Trace vitamins while gassing with N2. Separately autoclave Na2S x 9H2O as 5% solution under a N2 atmosphere. Aseptically and anaerobically add NaHCO3 and Trace vitamins to the medium under a N2-CO2 (90:10) atmosphere. Prior to inoculation, add the sterile Na2S x 9H2O solution, and adjust pH to 7.5 - 8.0.
Solution occurs in 2 other media (first defined in medium J231 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.640 g - -
2 6.200 g - -
3 0.550 g - -
4 1.000 g - -
5 0.100 g - -
6 0.170 g - -
7 0.130 g - -
8 0.180 g - -
9 0.050 g - -
10 0.018 g - -
11 0.010 g - -
12 0.011 g - -
13 0.025 g - -
Instructions:
  1. Dissolve EDTA x 2Na first in 500 ml of distilled water. Then add the remaining salts and adjust volume to 1000 ml.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 997.211 55353.5
NaCl 0.998 17.077
Yeast extract 0.998 -
NaHCO3 0.838 9.979
KCl 0.499 6.693
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