JCM 1064 . GEOSPOROBACTER MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
KCl 0.50 g 0.499 6.694
KH2PO4 0.20 g 0.2 1.467
NaCl 1.00 g 0.998 17.077
NH4Cl 0.05 g 0.05 0.933
CaCl2 x 2 H2O 0.10 g 0.1 0.679
MgCl2 x 6 H2O 0.40 g 0.399 1.964
Yeast extract 1.00 g 0.998 -
Glucose 0.36 g 0.359 1.994
Trace vitamins 1.00 ml - -
Trace element solution 1.00 ml - -
NaHCO3 0.84 g 0.838 9.979
Na2S x 9 H2O 0.50 g 0.499 2.078
Resazurin 1.00 mg 1.0e-3 0.004
Distilled water 1000.00 ml - -
1 Mix ingredients except NaHCO3, Trace vitamins and Na2S x 9H2O, and autoclave under a N2-CO2 (90:10) atmosphere. Filter-sterilize NaHCO3 as an 8.4% solution and Trace vitamins while gassing with N2. Separately autoclave Na2S x 9H2O as 5% solution under a N2 atmosphere. Aseptically and anaerobically add NaHCO3 and Trace vitamins to the medium under a N2-CO2 (90:10) atmosphere. Prior to inoculation, add the sterile Na2S x 9H2O solution, and adjust pH to 7.5 - 8.0.
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 -
Thiamine HCl 5.0 mg 0.005 -
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -
Solution occurs in 2 other media (first defined in Medium J231).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 0.640 g - -
MgSO4 x 7 H2O 6.200 g - -
MnSO4 x n H2O 0.550 g - -
NaCl 1.000 g - -
FeSO4 x 7 H2O 0.100 g - -
CoCl2 x 6 H2O 0.170 g - -
CaCl2 x 2 H2O 0.130 g - -
ZnSO4 x 7 H2O 0.180 g - -
CuSO4 x 5 H2O 0.050 g - -
AlK(SO4)2 x 12 H2O 0.018 g - -
H3BO3 0.010 g - -
Na2MoO4 x 2 H2O 0.011 g - -
NiCl2 x 6 H2O 0.025 g - -
1 Dissolve EDTA x 2Na first in 500 ml of distilled water. Then add the remaining salts and adjust volume to 1000 ml.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.8
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 0.998 17.077
Yeast extract 0.998 -
NaHCO3 0.838 9.979
KCl 0.499 6.694
Na2S x 9 H2O 0.499 2.078
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen anaerobe 1 strain
temperature thermophilic 2 strains