Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KCl | 0.50 | g | 0.499 | 6.694 | |
KH2PO4 | 0.20 | g | 0.2 | 1.467 | |
NaCl | 1.00 | g | 0.998 | 17.077 | |
NH4Cl | 0.05 | g | 0.05 | 0.933 | |
CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.679 | |
MgCl2 x 6 H2O | 0.40 | g | 0.399 | 1.964 | |
Yeast extract | 1.00 | g | 0.998 | - | |
Glucose | 0.36 | g | 0.359 | 1.994 | |
Trace vitamins | 1.00 | ml | - | - | |
Trace element solution | 1.00 | ml | - | - | |
NaHCO3 | 0.84 | g | 0.838 | 9.979 | |
Na2S x 9 H2O | 0.50 | g | 0.499 | 2.078 | |
Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
Distilled water | 1000.00 | ml | - | - | |
1 Mix ingredients except NaHCO3, Trace vitamins and Na2S x 9H2O, and autoclave under a N2-CO2 (90:10) atmosphere. Filter-sterilize NaHCO3 as an 8.4% solution and Trace vitamins while gassing with N2. Separately autoclave Na2S x 9H2O as 5% solution under a N2 atmosphere. Aseptically and anaerobically add NaHCO3 and Trace vitamins to the medium under a N2-CO2 (90:10) atmosphere. Prior to inoculation, add the sterile Na2S x 9H2O solution, and adjust pH to 7.5 - 8.0. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2-EDTA | 0.640 | g | - | - | |
MgSO4 x 7 H2O | 6.200 | g | - | - | |
MnSO4 x n H2O | 0.550 | g | - | - | |
NaCl | 1.000 | g | - | - | |
FeSO4 x 7 H2O | 0.100 | g | - | - | |
CoCl2 x 6 H2O | 0.170 | g | - | - | |
CaCl2 x 2 H2O | 0.130 | g | - | - | |
ZnSO4 x 7 H2O | 0.180 | g | - | - | |
CuSO4 x 5 H2O | 0.050 | g | - | - | |
AlK(SO4)2 x 12 H2O | 0.018 | g | - | - | |
H3BO3 | 0.010 | g | - | - | |
Na2MoO4 x 2 H2O | 0.011 | g | - | - | |
NiCl2 x 6 H2O | 0.025 | g | - | - | |
1 Dissolve EDTA x 2Na first in 500 ml of distilled water. Then add the remaining salts and adjust volume to 1000 ml. |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.8 |
Equipment needed: | Anaerobic workbench, Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 2 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 0.998 | 17.077 |
Yeast extract | 0.998 | - |
NaHCO3 | 0.838 | 9.979 |
KCl | 0.499 | 6.694 |
Na2S x 9 H2O | 0.499 | 2.078 |
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