JCM 1065 . ACIDIFIED MPOB MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.30 g 0.291 5.435
Na2SO4 1.42 g 1.376 9.687
CaCl2 x 2 H2O 0.11 g 0.107 0.725
MgCl2 x 6 H2O 0.10 g 0.097 0.477
NaCl 0.30 g 0.291 4.974
FeCl2 solution 1.00 ml - -
Trace element solution 1.00 ml - -
Yeast extract
(BD-Difco)
0.20 g 0.194 -
Trace vitamins 10.00 ml - -
Resazurin 0.50 mg 4.8e-4 0.002
Distilled water 1000.00 ml - -
1 Mix components thoroughly, bubble with a N2-CO2 (80:20, v/v) gas mixture, seal with a butyl rubber stopper and autoclave under the same gas mixture. After cooling, aseptically and anaerobically add per liter the following solutions (separately autoclaved under a N2 atmosphere):
K2HPO4
(1 M)
5.00 ml - -
glycerin
(1 M)
5.00 ml - -
L-Cysteine HCl x H2O
(5%)
10.00 ml - -
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 -
Thiamine HCl 5.0 mg 0.005 -
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 29.10.21
Source: JCM
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Anaerobic workbench, Autoclave
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2SO4 1.376 9.687
NH4Cl 0.291 5.435
NaCl 0.291 4.974
Yeast extract 0.194 -
CaCl2 x 2 H2O 0.107 0.725
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