Name: M25Y MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J1066 #

Equipment needed: Autoclave Anaerobic workbench Filter

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
MES	3.90	g	3.816	19.545
NaCl	25.00	g	24.462	418.58
MgSO₄ x 7 H₂O	2.70	g	2.642	10.719
MgCl₂ x 6 H₂O	4.30	g	4.207	20.695
NH₄Cl	0.25	g	0.245	4.573
KCl	0.34	g	0.333	4.463
CaCl₂ x 2 H₂O	0.14	g	0.137	0.932
K₂HPO₄	0.14	g	0.137	0.786
Yeast extract	0.20	g	0.196	-
Trace elements	1.00	ml	-	-
Fe(NH₄)₂(SO₄)₂ x 6 H₂O (in 0.037% HCl, 0.2%)	1.00	ml	-	-
Resazurin	1.00	mg	9.8e-4	0.004
Distilled water	1000.00	ml	-	-
1 Adjust pH to 5.5. Autoclave under a N₂ atmosphere. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter sterilized):
Trace vitamins (see Medium No. 197) (*)	10.00	ml	-	-
Na₂S x 9 H₂O (5%)	10.00	ml	-	-

Trace elements #

1000

Solution occurs in 2 other media (first defined in Medium J621).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Na₂-EDTA	29.00	g	29	-
MnSO₄ x n H₂O	3.26	g	3.26	-
CoCl₂ x 6 H₂O	1.80	g	1.8	13.863
ZnSO₄ x 7 H₂O	1.00	g	1	3.478
NiSO₄ x 6 H₂O	0.11	g	0.11	0.711
CuSO₄ x 5 H₂O	0.10	g	0.1	0.401
H₃BO₃	0.10	g	0.1	1.617
AlK(SO₄)₂ x 12 H₂O	0.10	g	0.1	-
Na₂MoO₄ x 2 H₂O	0.10	g	0.1	0.413
Na₂WO₄ x 2 H₂O	0.10	g	0.1	0.303
Na₂SeO₃	0.05	g	0.05	-
Distilled water	1000.00	ml	-	-
1 First dissolve EDTA x 2Na and adjust pH to 8.0 with KOH. Then add the minerals.

Last modified:	22.02.22
Source:	JCM
Taxonomic range:	Bacteria
Medium type:	Complex medium
Final pH:	5.5
Equipment needed:	Anaerobic workbench, Autoclave, Filter

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