JCM 1068 . SALTWATER IRON-OXIDIZING BACTERIA MEDIUM-II

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Artificial saltwater 1000.0 ml - -
Wolfe's mineral solution 10.0 ml - -
1 Mix components, distribute the medium into culture vessels (e.g., 10 ml in Balch-type tubes), seal with butyl rubber stoppers and autoclave. After autoclaving, aseptically add per liter the following solutions (filter-sterilized):
Trace vitamins 10.0 ml - -
NaHCO3
(8%)
12.5 ml - -
FeCl2
(freshly prepared, 0.2 M)
10.0 ml - -
2 Replace the gas phase with a N2-CO2-O2 (80:20:3, v/v/v) gas mixture and pressurize to 200 kPa with the same gas mixture.
Solution occurs in 3 other media (first defined in Medium J629).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 27.50 g 27.5 470.568
MgCl2 x 6 H2O 5.38 g 5.38 26.463
MgSO4 x 7 H2O 6.78 g 6.78 27.508
KCl 0.72 g 0.72 9.658
NaHCO3 0.20 g 0.2 2.381
CaCl2 x 2 H2O 1.40 g 1.4 9.523
NH4Cl 1.00 g 1 18.695
KH2PO4 0.05 g 0.05 0.367
Distilled water 1000.00 ml - -
Solution occurs in 7 other media (first defined in Medium J265).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trace minerals 1000.00 ml - -
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2SeO3 1.00 mg 1.0e-3 -
Na2WO4 x 2 H2O 0.01 g 0.01 0.03
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 -
Thiamine HCl 5.0 mg 0.005 -
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 26.401 451.765
MgSO4 x 7 H2O 6.536 26.516
MgCl2 x 6 H2O 5.163 25.396
CaCl2 x 2 H2O 1.345 9.146
NH4Cl 0.96 17.942
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
oxygen microaerophile 1 strain
temperature psychrophilic 1 strain