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J1068. SALTWATER IRON-OXIDIZING BACTERIA MEDIUM-II

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Artificial saltwater 1000.0 ml - -
2 Wolfe's mineral solution 10.0 ml - -
3 Trace vitamins 10.0 ml - -
4
(8%)
12.5 ml 2.072 24.665
5
(freshly prepared, 0.2 M)
10.0 ml 18.522 146.131
Instructions:
  1. Mix components, distribute the medium into culture vessels (e.g., 10 ml in Balch-type tubes), seal with butyl rubber stoppers and autoclave. After autoclaving, aseptically add per liter the following solutions (filter-sterilized): #3-5
  2. Replace the gas phase with a N2-CO2-O2 (80:20:3, v/v/v) gas mixture and pressurize to 200 kPa with the same gas mixture.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 7 other media (first defined in medium J265 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Trace minerals 1000.000 ml - -
2 0.020 g 0.02 -
3 0.001 g 1.0e-3 0.006
4 0.010 g 0.01 -
Solution occurs in 3 other media (first defined in medium J629 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 27.50 g 27.5 470.548
2 5.38 g 5.38 26.463
3 6.78 g 6.78 27.507
4 0.72 g 0.72 9.658
5 0.20 g 0.2 2.381
6 1.40 g 1.4 9.523
7 1.00 g 1 18.695
8 0.05 g 0.05 0.367
9 1000.00 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 977.132 54238.9
NaCl 26.401 451.746
FeCl2 18.522 146.131
MgSO4 x 7H2O 6.536 26.515
MgCl2 x 6H2O 5.163 25.397
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