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JCM 1068 . SALTWATER IRON-OXIDIZING BACTERIA MEDIUM-II

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1068 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Artificial saltwater 1000.0 ml - -
Wolfe's mineral solution 10.0 ml - -
1 Mix components, distribute the medium into culture vessels (e.g., 10 ml in Balch-type tubes), seal with butyl rubber stoppers and autoclave. After autoclaving, aseptically add per liter the following solutions (filter-sterilized):
Trace vitamins 10.0 ml - -
NaHCO3
(8%) 		12.5 ml - -
FeCl2
(freshly prepared, 0.2 M) 		10.0 ml - -
2 Replace the gas phase with a N2-CO2-O2 (80:20:3, v/v/v) gas mixture and pressurize to 200 kPa with the same gas mixture.

Artificial saltwater #

1000
Solution occurs in 3 other media (first defined in Medium J629).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 27.50 g 27.5 470.568
MgCl2 x 6 H2O 5.38 g 5.38 26.463
MgSO4 x 7 H2O 6.78 g 6.78 27.508
KCl 0.72 g 0.72 9.658
NaHCO3 0.20 g 0.2 2.381
CaCl2 x 2 H2O 1.40 g 1.4 9.523
NH4Cl 1.00 g 1 18.695
KH2PO4 0.05 g 0.05 0.367
Distilled water 1000.00 ml - -

Wolfe's mineral solution #

1000
Solution occurs in 7 other media (first defined in Medium J265).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trace minerals 1000.00 ml - -
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2SeO3 1.00 mg 1.0e-3 -
Na2WO4 x 2 H2O 0.01 g 0.01 0.03

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Trace minerals #

1000
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 26.401 451.765
MgSO4 x 7 H2O 6.536 26.516
MgCl2 x 6 H2O 5.163 25.396
CaCl2 x 2 H2O 1.345 9.146
NH4Cl 0.96 17.942
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
oxygen microaerophile 1 strain
temperature psychrophilic 1 strain