1069 .
MICROAEROPHILIC LUTIBACTER MEDIUM
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Equipment needed:
Autoclave
Final pH:
6.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Artificial saltwater | 1000.00 | ml | ||
|
|
MES | 1.95 | g | ||
|
|
Wolfe's mineral solution | 1.00 | ml | ||
|
|
Trace vitamins | 10.00 | ml | ||
|
|
Tryptone
(BD-Difco) |
2.00 | g | ||
| 1 Mix components and adjust pH to 6.2 to 6.5. Distribute the medium into culture vessels, replace the gas phase with N2-CO2-O2 (90:10:2, v/v/v), seal with butyl rubber stoppers and autoclave. | |||||
Artificial saltwater #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NaCl | 27.50 | g |
|
|
MgCl2 x 6 H2O | 5.38 | g |
|
|
MgSO4 x 7 H2O | 6.78 | g |
|
|
KCl | 0.72 | g |
|
|
NaHCO3 | 0.20 | g |
|
|
CaCl2 x 2 H2O | 1.40 | g |
|
|
NH4Cl | 1.00 | g |
|
|
KH2PO4 | 0.05 | g |
|
|
Distilled water | 1000.00 | ml |
Wolfe's mineral solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Trace minerals | 1000.00 | ml |
|
|
NiCl2 x 6 H2O | 0.02 | g |
|
|
Na2SeO3 | 1.00 | mg |
|
|
Na2WO4 x 2 H2O | 0.01 | g |
Trace vitamins #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
|
Folic acid | 2.0 | mg |
|
|
Pyridoxine hydrochloride | 10.0 | mg |
|
|
Thiamine HCl | 5.0 | mg |
|
|
Riboflavin | 5.0 | mg |
|
|
Nicotinic acid | 5.0 | mg |
|
|
Calcium pantothenate | 5.0 | mg |
|
|
Vitamin B12 | 0.1 | mg |
|
|
p-Aminobenzoic acid | 5.0 | mg |
|
|
Lipoic acid | 5.0 | mg |
|
|
Distilled water | 1000.0 | ml |
Trace minerals #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nitrilotriacetic acid | 1.50 | g | ||
|
|
MgSO4 x 7 H2O | 3.00 | g | ||
|
|
MnSO4 x n H2O | 0.50 | g | ||
|
|
NaCl | 1.00 | g | ||
|
|
FeSO4 x 7 H2O | 0.10 | g | ||
|
|
CoSO4 x 7 H2O | 0.10 | g | ||
|
|
CaCl2 x 2 H2O | 0.10 | g | ||
|
|
ZnSO4 x 7 H2O | 0.10 | g | ||
|
|
CuSO4 x 5 H2O | 0.01 | g | ||
|
|
AlK(SO4)2 | 0.01 | g | ||
|
|
H3BO3 | 0.01 | g | ||
|
|
Na2MoO4 x 2 H2O | 0.01 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. | |||||