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JCM 1070 . MO-XQ MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1070 #

ml
Equipment needed: Anaerobic workbench Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract
(BD-Difco) 		1.000 g 0.899 -
NH4Cl 0.535 g 0.481 8.994
KH2PO4 0.136 g 0.122 0.899
MgCl2 x 6 H2O 3.050 g 2.743 13.491
CaCl2 x 2 H2O 0.147 g 0.132 0.899
NaCl 20.450 g 18.39 314.687
NaHCO3 2.520 g 2.266 26.976
Trace vitamins solution 1.000 ml - -
Trace element solution 1.000 ml - -
Resazurin 1.000 mg 9.0e-4 0.004
Distilled water 1000.000 ml - -
1 Solution B:
Glucose 7.200 g 6.475 35.939
Distilled water 100.000 ml - -
2 To complete the medium, add 0.1 volume of solution B to the culture vessels containing solution A. Prior to inoculation, aseptically and anaerobically add per liter the following solutions (autoclaved and stored under a N2 atmosphere):
L-Cysteine HCl x H2O
(3%) 		5.000 ml - -
Na2S x 9 H2O
(3%) 		5.000 ml - -
3 Solution A:
4 Mix components, except NaHCO3, and adjust pH to 7.5. Bring to a boil for 5-10 sec., then, cool down under a stream of N2-CO2 (80:20, v/v) and add NaHCO3 to the solution. Dispense 0.9 volume of the medium into culture vessels (e.g., 18 ml of the medium in 50 ml serum bottle) under a stream of N2-CO2 (80:20, v/v) and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight.
5 Adjust pH to 7.5 and autoclave under a N2 atmosphere.

Trace vitamins solution #

1000
Solution occurs in 3 other media (first defined in Medium J682).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 4.9 mg 0.005 0.02
Folic acid 8.8 mg 0.009 0.02
Pyridoxine hydrochloride 4.1 mg 0.004 -
Thiamine HCl 6.7 mg 0.007 -
Riboflavin 7.5 mg 0.008 0.02
Nicotinic acid 2.5 mg 0.003 0.02
DL-Calcium pantothenate 9.5 mg 0.01 0.02
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 2.7 mg 0.003 0.02
Lipoic acid 4.1 mg 0.004 0.02
Distilled water 1000.0 ml - -

Trace element solution #

1000
Solution occurs in 2 other media (first defined in Medium J682).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeCl3 x 6 H2O 1.27 g 1.27 4.699
MnCl2 x 4 H2O 0.20 g 0.2 -
CoCl2 x 6 H2O 0.13 g 0.13 1.001
ZnCl2 0.14 g 0.14 1.027
CuCl2 x 2 H2O 1.00 mg 1.0e-3 0.006
AlCl3 0.01 g 0.01 0.075
H3BO3 6.00 mg 0.006 0.097
Na2MoO4 x 2 H2O 0.02 g 0.02 0.083
NiCl2 x 6 H2O 0.01 g 0.01 0.077
Na2SeO3 2.00 mg 0.002 -
Na2WO4 x 2 H2O 3.00 mg 0.003 0.009
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Anaerobic workbench, Autoclave
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 18.39 314.687
Glucose 6.475 35.939
MgCl2 x 6 H2O 2.743 13.491
NaHCO3 2.266 26.976
Yeast extract 0.899 -
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