Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2SO4 | 3.00 | g | 2.947 | 20.747 | |
KH2PO4 | 0.20 | g | 0.196 | 1.444 | |
NH4Cl | 0.30 | g | 0.295 | 5.509 | |
NaCl | 13.50 | g | 13.261 | 226.922 | |
MgCl2 x 6 H2O | 2.20 | g | 2.161 | 10.63 | |
KCl | 0.50 | g | 0.491 | 6.588 | |
CaCl2 x 2 H2O | 0.15 | g | 0.147 | 1.002 | |
Na2SeO3 | 3.00 | μg | - | - | |
FeCl2 solution | 1.00 | ml | - | - | |
Trace element solution | 1.00 | ml | - | - | |
Trace vitamins | 10.00 | ml | - | - | |
Yeast extract | 0.50 | g | 0.491 | - | |
Resazurin | 1.00 | mg | 9.8e-4 | 0.004 | |
Distilled water | 950.00 | ml | - | - | |
1 Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) atmosphere. After cooling, add the following solutions (autoclaved or *filter-sterilized): | |||||
NaHCO3
(*, 8%) |
30.00 | ml | - | - | |
L-Sodium lactate
(10%) |
20.00 | ml | - | - | |
2 Aseptically distribute the medium into culture vessels under and a N2-CO2 (4:1, v/v) gas stream and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solution (autoclaved and stored under a N2 atmosphere): | |||||
Na2S x 9 H2O
(5%) |
6.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
HCl
(7.7 M, 25%) |
10.0 | ml | 2.5 | 68.568 |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 |
Distilled water | 990.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
ZnCl2 | 70 | mg | 0.07 | 0.514 |
MnCl2 x 4 H2O | 100 | mg | 0.1 | 0.505 |
H3BO3 | 6 | mg | 0.006 | 0.097 |
CoCl2 x 6 H2O | 190 | mg | 0.19 | 1.463 |
CuCl2 x 2 H2O | 2 | mg | 0.002 | 0.012 |
NiCl2 x 6 H2O | 24 | mg | 0.024 | 0.185 |
Na2MoO4 x 2 H2O | 36 | mg | 0.036 | 0.149 |
Distilled water | 1000 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 29.10.21 |
Source: | JCM |
Medium type: | Complex medium |
Final pH: | n.d. |
Equipment needed: | Autoclave, Filter |
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