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JCM 1074 . VULCANIBACILLUS B5 MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1074 #

ml
Equipment needed: Filter Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.5 g 0.49 9.164
KCl 0.5 g 0.49 6.575
KH2PO4 0.2 g 0.196 1.441
CaCl2 x 2 H2O 0.1 g 0.098 0.667
MgCl2 x 6 H2O 0.4 g 0.392 1.929
NaCl 1.0 g 0.98 16.776
NaNO3 3.0 g 2.941 34.604
Yeast extract 1.0 g 0.98 -
Trace vitamins 10.0 ml - -
Trace element solution 10.0 ml - -
NaHCO3 2.5 g 2.451 29.176
Na2S x 9 H2O 0.3 g 0.294 1.225
Resazurin 1.0 mg 9.8e-4 0.004
Distilled water 1000.0 ml - -
1 Mix ingredients except NaHCO3, Trace vitamins and Na2S x 9H2O, and autoclave under a N2-CO2 (90:10) atmosphere. Filter-sterilize NaHCO3 as an 8.3% solution and Trace vitamins while gassing with N2. Separately autoclave Na2S x 9H2O as a 3% solution under a N2 atmosphere. Aseptically and anaerobically add NaHCO3 and Trace vitamins to the medium under a N2-CO2 (90:10) atmosphere. Prior to inoculation, add the sterile Na2S x 9H2O solution and adjust pH to 7.0.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Trace element solution #

Solution occurs in 2 other media (first defined in Medium J231).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 0.640 g - -
MgSO4 x 7 H2O 6.200 g - -
MnSO4 x n H2O 0.550 g - -
NaCl 1.000 g - -
FeSO4 x 7 H2O 0.100 g - -
CoCl2 x 6 H2O 0.170 g - -
CaCl2 x 2 H2O 0.130 g - -
ZnSO4 x 7 H2O 0.180 g - -
CuSO4 x 5 H2O 0.050 g - -
AlK(SO4)2 x 12 H2O 0.018 g - -
H3BO3 0.010 g - -
Na2MoO4 x 2 H2O 0.011 g - -
NiCl2 x 6 H2O 0.025 g - -
1 Dissolve EDTA x 2Na first in 500 ml of distilled water. Then add the remaining salts and adjust volume to 1000 ml.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaNO3 2.941 34.604
NaHCO3 2.451 29.176
NaCl 0.98 16.776
Yeast extract 0.98 -
NH4Cl 0.49 9.164
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain