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JCM 1075 . CUNICULIPLASMA MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1075 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 1.30 g 1.286 9.731
KH2PO4 0.28 g 0.277 2.035
MgSO4 x 7 H2O 0.25 g 0.247 1.003
CaCl2 x 2 H2O 0.07 g 0.069 0.471
FeCl3 x 6 H2O 0.02 mg 2.0e-5 7.3e-5
Beef extract
(BD-Difco) 		1.00 g 0.989 -
Yeast extract 0.10 g 0.099 -
Tryptone 1.00 g 0.989 13.916
Trace element solution 1.00 ml - -
Kao and Michayluk vitamin solution
(Sigma-Aldrich) 		10.00 ml - -
Betaine 0.60 g 0.593 5.066
Distilled water 1000.00 ml - -
1 Adjust pH to 1.0 - 1.2 with H2SO4.

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 1.1
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
(NH4)2SO4 1.286 9.731
Tryptone 0.989 13.916
Beef extract 0.989 -
Betaine 0.593 5.066
KH2PO4 0.277 2.035
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Cultivation metadata from 2 strains BacDive

pH 0 - 2 1 strain
phyla 1 2 strains
nutrition organoheterotroph 1 strain
oxygen facultative anaerobe 1 strain
temperature mesophilic 2 strains