JCM 1077 . ALKALIPHILUS LACV MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 10.0 g 9.93 169.926
NH4Cl 1.0 g 0.993 18.565
KH2PO4 0.1 g 0.099 0.73
K2HPO4 0.1 g 0.099 0.57
KCl 0.1 g 0.099 1.332
MgCl2 x 6 H2O 0.1 g 0.099 0.488
Tris base 3.0 g 2.979 24.593
Na2S2O3 x 5 H2O 3.0 g 2.979 12.004
Trace element solution 1.0 ml - -
Resazurin 1.0 mg 9.9e-4 0.004
Distilled water 940.0 ml - -
1 Mix components thoroughly, adjust pH to 9.0 with NaOH and autoclave under a N2 atmosphere. After cooling, replace the gas phase with a N2-CO2 (4:1, v/v) gas mixture. Add the following solutions from sterile anaerobic stocks (autoclaved and stored under a N2 atmosphere):
Yeast extract
(w/v, 10%)
20.0 ml - -
Crotonic acid
(pH 7.0, 0.5 M)
20.0 ml - -
Na2CO3
(8%)
20.0 ml - -
Na2S x 9 H2O
(5%)
6.0 ml - -
2 Check final pH be around 8.5.
Solution occurs in 38 other media (first defined in Medium J439).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
12.5 ml - -
FeSO4 x 7 H2O 2.1 g 2.1 7.554
H3BO3 30.0 mg 0.03 0.485
MnCl2 x 4 H2O 100.0 mg 0.1 -
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
ZnSO4 x 7 H2O 144.0 mg 0.144 0.501
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 987.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.5
Equipment needed: Anaerobic workbench, Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 9.93 169.926
Tris base 2.979 24.593
Na2S2O3 x 5 H2O 2.979 12.004
NH4Cl 0.993 18.565
KCl 0.099 1.332
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Cultivation metadata from 1 strains BacDive

pH 8 - 10 1 strain
phyla 1 1 strain
nutrition organoheterotroph 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain