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JCM 1078 . ALKALIPHILUS 3B MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 1.0 g 0.993 16.993
NH4Cl 1.0 g 0.993 18.565
KH2PO4 0.1 g 0.099 0.73
K2HPO4 0.1 g 0.099 0.57
KCl 0.1 g 0.099 1.332
MgCl2 x 6 H2O 0.1 g 0.099 0.488
Tris base 3.0 g 2.979 24.593
Na2S2O3 x 5 H2O 1.5 g 1.49 6.002
Trace element solution 1.0 ml - -
Resazurin 1.0 mg 9.9e-4 0.004
Distilled water 910.0 ml - -
1 Mix components thoroughly, adjust pH to 9.0 with NaOH and autoclave under a N2 atmosphere. After cooling, replace the gas phase with a N2-CO2 (4:1, v/v) gas mixture. Add the following solutions from sterile anaerobic stocks (autoclaved and stored under a N2 atmosphere):
Yeast extract
(w/v, 10%)
50.0 ml - -
Crotonic acid
(pH 7.0, 0.5 M)
20.0 ml - -
Na2CO3
(8%)
20.0 ml - -
Na2S x 9 H2O
(5%)
6.0 ml - -
2 Check final pH be around 8.5.
Solution occurs in 38 other media (first defined in Medium J439).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
12.5 ml - -
FeSO4 x 7 H2O 2.1 g 2.1 7.554
H3BO3 30.0 mg 0.03 0.485
MnCl2 x 4 H2O 100.0 mg 0.1 -
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
ZnSO4 x 7 H2O 144.0 mg 0.144 0.501
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 987.0 ml - -

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.5
Equipment needed: Anaerobic workbench, Autoclave

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Tris base 2.979 24.593
Na2S2O3 x 5 H2O 1.49 6.002
NH4Cl 0.993 18.565
NaCl 0.993 16.993
KCl 0.099 1.332
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
pH 10 - 12 1 strain
phyla 1 1 strain
oxygen obligate anaerobe 1 strain
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