Name: ALKALIPHILUS 3B MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J1078 #

Equipment needed: Autoclave Anaerobic workbench

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NaCl	1.0	g	0.993	16.993
NH₄Cl	1.0	g	0.993	18.565
KH₂PO₄	0.1	g	0.099	0.73
K₂HPO₄	0.1	g	0.099	0.57
KCl	0.1	g	0.099	1.332
MgCl₂ x 6 H₂O	0.1	g	0.099	0.488
Tris base	3.0	g	2.979	24.593
Na₂S₂O₃ x 5 H₂O	1.5	g	1.49	6.002
Trace element solution	1.0	ml	-	-
Resazurin	1.0	mg	9.9e-4	0.004
Distilled water	910.0	ml	-	-
1 Mix components thoroughly, adjust pH to 9.0 with NaOH and autoclave under a N₂ atmosphere. After cooling, replace the gas phase with a N₂-CO₂ (4:1, v/v) gas mixture. Add the following solutions from sterile anaerobic stocks (autoclaved and stored under a N₂ atmosphere):
Yeast extract (w/v, 10%)	50.0	ml	-	-
Crotonic acid (pH 7.0, 0.5 M)	20.0	ml	-	-
Na₂CO₃ (8%)	20.0	ml	-	-
Na₂S x 9 H₂O (5%)	6.0	ml	-	-
2 Check final pH be around 8.5.

Trace element solution #

1000

Solution occurs in 38 other media (first defined in Medium J439).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
HCl (25%)	12.5	ml	-	-
FeSO₄ x 7 H₂O	2.1	g	2.1	7.554
H₃BO₃	30.0	mg	0.03	0.485
MnCl₂ x 4 H₂O	100.0	mg	0.1	-
CoCl₂ x 6 H₂O	190.0	mg	0.19	1.463
NiCl₂ x 6 H₂O	24.0	mg	0.024	0.185
CuCl₂ x 2 H₂O	2.0	mg	0.002	0.012
ZnSO₄ x 7 H₂O	144.0	mg	0.144	0.501
Na₂MoO₄ x 2 H₂O	36.0	mg	0.036	0.149
Distilled water	987.0	ml	-	-

Last modified:	22.02.22
Source:	JCM
Taxonomic range:	Bacteria
Medium type:	Complex medium
Final pH:	8.5
Equipment needed:	Anaerobic workbench, Autoclave

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