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JCM 1079 . HALANAEROARCHAEUM MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 240.0 g 8571.43 146671
K2HPO4 2.5 g 89.286 512.618
NH4Cl 0.5 g 17.857 333.841
HEPES 7.0 g 250 1049.05
1 Add components to distilled water and bring volume to 1.0 L. Autoclave and, after cooling, add per liter the following solutions (autoclaved or *filter-sterilized separately):
MgCl2
(1 M)
1.0 ml - -
Selenite-tungstate solution 1.0 ml - -
Trace element solution 1.0 ml - -
NaHCO3
(*, 1 M)
10.0 ml - -
2 To complete the medium, aseptically and anaerobically distribute the medium into culture vessels containing steam-sterilized sulfur (see below, approximately 1.0 g/L) under an argon gas stream, and seal with butyl rubber stoppers. Then, add per liter the following solutions (autoclaved or *filter-sterilized):
Sodium pyruvate
(*, 2 M)
10.0 ml - -
Yeast extract
(10%)
0.2 ml - -
Na2S x 9 H2O
(5%)
5.0 ml - -
3 Basal mineral NaCl medium:
Solution occurs in 4 other media (first defined in Medium J1079).
Equipment needed: Autoclave
1 Steam sulfur for 3 hr, or autoclave at 105C for 1 hr, on each of 3 successive days.
Solution occurs in 13 other media (first defined in Medium J1079).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.00 g 2 7.194
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 -
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 Adjust pH to 3.6.
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 3.6
Equipment needed: Anaerobic workbench, Autoclave, Filter

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 8571.43 146671
HEPES 250 1049.05
K2HPO4 89.286 512.618
NH4Cl 17.857 333.841
EDTA 0.179 0.611
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Cultivation metadata from 1 strains BacDive

halophily extremely halophilic 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
oxygen obligate anaerobe 1 strain
temperature mesophilic 1 strain
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