J1079
1079 .
HALANAEROARCHAEUM MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1079 #
ml
Equipment needed: Autoclave
Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 240.0 | g | 8571.43 | 146671 | |
| K2HPO4 | 2.5 | g | 89.286 | 512.618 | |
| NH4Cl | 0.5 | g | 17.857 | 333.841 | |
| HEPES | 7.0 | g | 250 | 1049.05 | |
| 1 Add components to distilled water and bring volume to 1.0 L. Autoclave and, after cooling, add per liter the following solutions (autoclaved or *filter-sterilized separately): | |||||
|
MgCl2
(1 M) |
1.0 | ml | - | - | |
| Selenite-tungstate solution | 1.0 | ml | - | - | |
| Trace element solution | 1.0 | ml | - | - | |
|
NaHCO3
(*, 1 M) |
10.0 | ml | - | - | |
| 2 To complete the medium, aseptically and anaerobically distribute the medium into culture vessels containing steam-sterilized sulfur (see below, approximately 1.0 g/L) under an argon gas stream, and seal with butyl rubber stoppers. Then, add per liter the following solutions (autoclaved or *filter-sterilized): | |||||
|
Sodium pyruvate
(*, 2 M) |
10.0 | ml | - | - | |
|
Yeast extract
(10%) |
0.2 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
5.0 | ml | - | - | |
| 3 Basal mineral NaCl medium: | |||||
Solution occurs in 4
other media (first defined in Medium J1079).
Equipment needed: Autoclave
| 1 Steam sulfur for 3 hr, or autoclave at 105C for 1 hr, on each of 3 successive days. |
Solution occurs in 13
other media (first defined in Medium J1079).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| EDTA | 5.00 | g | 5 | 17.109 | |
| FeSO4 x 7 H2O | 2.00 | g | 2 | 7.194 | |
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
| MnCl2 x 4 H2O | 0.03 | g | 0.03 | - | |
| H3BO3 | 0.30 | g | 0.3 | 4.851 | |
| CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 | |
| CuCl2 x 2 H2O | 0.01 | g | 0.01 | 0.059 | |
| NiCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 | |
| Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 3.6. | |||||
Solution occurs in 71
other media (first defined in Medium J431).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaOH | 0.4 | g | 0.4 | 10.001 |
| Na2SeO3 x 5 H2O | 6.0 | mg | 0.006 | 0.023 |
| Na2WO4 x 2 H2O | 8.0 | mg | 0.008 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Archaea |
| Medium type: | Complex medium |
| Final pH: | 3.6 |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 8571.43 | 146671 |
| HEPES | 250 | 1049.05 |
| K2HPO4 | 89.286 | 512.618 |
| NH4Cl | 17.857 | 333.841 |
| EDTA | 0.179 | 0.611 |
| Show more | ||
