Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Basal mineral NaCl medium
(sterilized, see Medium No. 1079) |
937.5 | ml | - | - | |
Soda based mineral medium
(sterilized) |
62.5 | ml | - | - | |
1 To complete the medium, aseptically add the following solution (autoclaved or *filter-sterilized): | |||||
Sodium pyruvate
(*, 2 M) |
5.0 | ml | - | - | |
Trace vitamins (see Medium No. 197)
(*) |
10.0 | ml | - | - | |
Yeast extract
(10%) |
0.2 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2CO3 | 185 | g | 46250 | 436370 | |
NaHCO3 | 35 | g | 8750 | 104158 | |
NaCl | 16 | g | 4000 | 68446.3 | |
K2HPO4 | 1 | g | 250 | 1435.33 | |
KCl | 5 | g | 1250 | 16767.3 | |
1 Add components to distilled water and bring volume to 1.0 L. Autoclave and let it stand for three days. Decant the clear solution into another empty sterile bottle. Aseptically add per liter the following solutions (autoclaved): | |||||
NH4Cl
(4 M) |
1 | ml | - | - | |
Selenite-tungstate solution | 1 | ml | - | - | |
Trace element solution | 1 | ml | - | - | |
MgCl2
(1 M) |
1 | ml | - | - |
Last modified: | 29.10.21 |
Source: | JCM |
Taxonomic range: | Archaea |
Medium type: | Complex medium |
Final pH: | n.d. |
Equipment needed: | Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Na2CO3 | 2847.91 | 26870.1 |
NaHCO3 | 538.793 | 6413.67 |
NaCl | 246.305 | 4214.67 |
KCl | 76.97 | 1032.47 |
K2HPO4 | 15.394 | 88.382 |
Show more |