Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2HPO4 x 2 H2O | 0.53 | g | 0.519 | 3.657 | |
KH2PO4 | 0.41 | g | 0.402 | 2.951 | |
NH4Cl | 0.30 | g | 0.294 | 5.493 | |
NaCl | 0.30 | g | 0.294 | 5.028 | |
MgCl2 x 6 H2O | 0.10 | g | 0.098 | 0.482 | |
CaCl2 x 2 H2O | 0.11 | g | 0.108 | 0.733 | |
Acid trace element solution | 1.00 | ml | - | - | |
Alkaline trace element solution | 1.00 | ml | - | - | |
Sodium acetate x 3 H2O | 0.68 | g | 0.666 | - | |
Yeast extract | 0.10 | g | 0.098 | - | |
Sulfur
(powder) |
0.80 | g | 0.784 | 24.432 | |
Resazurin | 1.00 | m | - | - | |
Distilled water | 1000.00 | ml | - | - | |
1 Mix components, except sulfur, and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions (filter-sterilized): | |||||
Vitamin solution | 0.20 | ml | - | - | |
NaHCO3
(8%) |
12.50 | ml | - | - | |
2 Steam sulfur for 3 hr on each of 3 successive days. Aseptically and anaerobically distribute the medium and sulfur into culture vessels (e.g., 50 ml of the medium and 0.04 g of sulfur in 120 ml serum bottle) under a N2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Prior to use, anaerobically add per liter the following solution from an anaerobic stock (autoclaved and stored under a N2 atmosphere): | |||||
Na2S x 9 H2O
(5%) |
6.00 | ml | - | - | |
3 Adjust pH to 6.0, if necessary. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
HCl
(6 N) |
10.00 | ml | - | - |
H3BO3 | 61.80 | mg | 0.061 | 0.989 |
MnCl2 x 4 H2O | 61.25 | mg | 0.061 | - |
FeCl2 x 4 H2O | 943.50 | mg | 0.934 | 4.699 |
CoCl2 x 6 H2O | 64.50 | mg | 0.064 | 0.492 |
NiCl2 x 6 H2O | 12.86 | mg | 0.013 | 0.098 |
ZnCl2 | 67.70 | mg | 0.067 | 0.492 |
Distilled water | 1000.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaOH | 400.0 | mg | 0.4 | 10.001 |
Na2SeO3 x 5 H2O | 17.3 | mg | 0.017 | 0.066 |
Na2WO4 x 2 H2O | 29.4 | mg | 0.029 | 0.089 |
Na2MoO4 x 2 H2O | 20.5 | mg | 0.021 | 0.085 |
Distilled water | 1000.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Biotin | 20 | mg | 0.02 | 0.082 | |
Nicotinic acid | 200 | mg | 0.2 | 1.625 | |
p-Aminobenzoic acid | 100 | mg | 0.1 | 0.729 | |
Thiamine HCl | 200 | mg | 0.2 | - | |
DL-Calcium pantothenate | 100 | mg | 0.1 | 0.21 | |
Pyridoxine hydrochloride | 500 | mg | 0.5 | - | |
Vitamin B12 | 100 | mg | 0.1 | 0.074 | |
Riboflavin | 100 | mg | 0.1 | 0.266 | |
Distilled water | 1000 | ml | - | - | |
1 Filter-sterilize and store under a N2 atmosphere. |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 6.0 |
Equipment needed: | Anaerobic workbench, Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Sulfur | 0.784 | 24.432 |
Sodium acetate x 3 H2O | 0.666 | - |
Na2HPO4 x 2 H2O | 0.519 | 3.657 |
KH2PO4 | 0.402 | 2.951 |
NH4Cl | 0.294 | 5.493 |
Show more |