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JCM 1090 . MMJS MEDIUM 2

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 20.00 g 19.608 335.521
K2HPO4 0.14 g 0.137 0.788
CaCl2 x 2 H2O 1.00 g 0.98 6.669
MgSO4 x 7 H2O 3.40 g 3.333 13.524
MgCl2 x 6 H2O 4.18 g 4.098 20.157
NH4Cl 0.25 g 0.245 4.582
KCl 0.33 g 0.324 4.34
NiCl2 x 6 H2O 0.50 mg 4.9e-4 0.004
Na2SeO3 x 5 H2O 0.50 mg 4.9e-4 0.002
Na2WO4 x 2 H2O 0.10 mg 9.8e-5 3.0e-4
(NH4)2Ni(SO4)2 x 6 H2O 10.00 mg 0.01 0.025
Trace minerals 10.00 ml - -
Distilled water 1000.00 ml - -
1 Mix components and adjust pH to 5.5. Distribute the medium into culture vessels (e.g., 20 ml in 60 ml-serum bottles) under a stream of N2, seal with butyl rubber stoppers and add Autoclave. After cooling, add per liter the following solutions (filter-sterilized):
Trace vitamins 1.00 ml - -
NaHCO3
(8%)
3.75 ml - -
Na2S2O3
(1 M)
5.00 ml - -
2 Replace the gas phase with N2-CO2-O2 (94:5:1, v/v/v, 100 kPa). Check final pH to be around 6.2.
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 -
Thiamine HCl 5.0 mg 0.005 -
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.2
Equipment needed: Autoclave, Filter

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 19.618 335.689
MgCl2 x 6 H2O 4.098 20.157
MgSO4 x 7 H2O 3.363 13.643
CaCl2 x 2 H2O 0.981 6.675
KCl 0.324 4.34
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
phyla 1 1 strain
nutrition chemolithoheterotroph 1 strain
oxygen obligate aerobe 1 strain
temperature mesophilic 1 strain

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