Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 20.00 | g | 19.608 | 335.521 | |
K2HPO4 | 0.14 | g | 0.137 | 0.788 | |
CaCl2 x 2 H2O | 1.00 | g | 0.98 | 6.669 | |
MgSO4 x 7 H2O | 3.40 | g | 3.333 | 13.524 | |
MgCl2 x 6 H2O | 4.18 | g | 4.098 | 20.157 | |
NH4Cl | 0.25 | g | 0.245 | 4.582 | |
KCl | 0.33 | g | 0.324 | 4.34 | |
NiCl2 x 6 H2O | 0.50 | mg | 4.9e-4 | 0.004 | |
Na2SeO3 x 5 H2O | 0.50 | mg | 4.9e-4 | 0.002 | |
Na2WO4 x 2 H2O | 0.10 | mg | 9.8e-5 | 3.0e-4 | |
(NH4)2Ni(SO4)2 x 6 H2O | 10.00 | mg | 0.01 | 0.025 | |
Trace minerals | 10.00 | ml | - | - | |
Distilled water | 1000.00 | ml | - | - | |
1 Mix components and adjust pH to 5.5. Distribute the medium into culture vessels (e.g., 20 ml in 60 ml-serum bottles) under a stream of N2, seal with butyl rubber stoppers and add Autoclave. After cooling, add per liter the following solutions (filter-sterilized): | |||||
Trace vitamins | 1.00 | ml | - | - | |
NaHCO3
(8%) |
3.75 | ml | - | - | |
Na2S2O3
(1 M) |
5.00 | ml | - | - | |
2 Replace the gas phase with N2-CO2-O2 (94:5:1, v/v/v, 100 kPa). Check final pH to be around 6.2. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
NaCl | 1.00 | g | 1 | 17.112 | |
FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
CoSO4 x 7 H2O | 0.10 | g | 0.1 | 0.645 | |
CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
AlK(SO4)2 | 0.01 | g | 0.01 | 0.039 | |
H3BO3 | 0.01 | g | 0.01 | 0.162 | |
Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | 6.2 |
Equipment needed: | Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 19.618 | 335.689 |
MgCl2 x 6 H2O | 4.098 | 20.157 |
MgSO4 x 7 H2O | 3.363 | 13.643 |
CaCl2 x 2 H2O | 0.981 | 6.675 |
KCl | 0.324 | 4.34 |
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