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JCM 1093 . MJ BASAL MEDIUM WITH THIOSULFATE/2

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1093 #

ml
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
MJ(-N) synthetic seawater 1000.00 ml - -
NH4Cl 0.25 g 0.123 2.307
KNO3 0.25 g 0.123 1.221
Distilled water 1000.00 ml - -
1 Mix components thoroughly and autoclave. After cooling, add the following solutions (filter-sterilized):
NaHCO3
(8%) 		10.00 ml - -
Na2S2O3 x 5 H2O
(10%) 		15.00 ml - -
Trace vitamins 1.00 ml - -
2 Aseptically distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) and seal with butyl rubber stoppers. Replace the gas phase with a N2-CO2 (80:20, v/v) gas mixture, and pressurize to 200 kPa. Adjust pH to 6.0, if necessary.

MJ(-N) synthetic seawater #

1010
Solution occurs in 12 other media (first defined in Medium J268).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 30.00 g 29.703 508.264
K2HPO4 0.14 g 0.139 0.796
CaCl2 x 2 H2O 0.14 g 0.139 0.943
MgSO4 x 7 H2O 3.40 g 3.366 13.658
MgCl2 x 6 H2O 4.18 g 4.139 20.357
KCl 0.33 g 0.327 4.383
NiCl2 x 6 H2O 0.50 mg 5.0e-4 0.004
Na2SeO3 x 5 H2O 0.50 mg 5.0e-4 0.002
Fe(NH4)2(SO4)2 x 6 H2O 0.01 g 0.01 -
Modified Wolin's mineral solution 10.00 ml - -
Distilled water 1000.00 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Trace minerals #

1000
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.0
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 14.666 250.954
MgCl2 x 6 H2O 2.043 10.048
MgSO4 x 7 H2O 1.676 6.801
KCl 0.161 2.163
NH4Cl 0.123 2.307
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain