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J1095. BICARBONATE BUFFERED MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.53 g 0.523 -
2 0.41 g 0.404 2.971
3 0.30 g 0.296 5.531
4 0.30 g 0.296 5.062
5 FeCl2 solution 1.00 ml - -
6 Trace element solution 1.00 ml - -
7 Selenite-tungstate solution 0.50 ml - -
8 0.10 g 0.099 -
9 0.50 mg 4.9e-4 0.002
10 930.00 ml 915.515 50818.8
11
(10%)
1.00 ml - -
12
(10%)
1.00 ml - -
13 Trace vitamins
(*)
10.00 ml - -
14
(*, 8%)
50.00 ml 8.517 101.382
15 Substrate Solution 10.00 ml - -
16
(5%)
10.00 ml - -
Instructions:
  1. Mix components and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions (autoclaved or *filter-sterilized): #11-15
  2. Aseptically and anaerobically distribute the medium into culture vessels (e.g., 50 ml of the medium in 120 ml serum bottle) under a N2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Prior to use, add per liter the following solution (autoclaved and stored under a N2 atmosphere): #16
Instructions:
  1. For strain JCM 30818, 1 M glycerin solution;
  2. For strain JCM 31247, 1 M trisodium citrate solution;
  3. For strain JCM 31248, 1 M maltose solution.
Solution occurs in 89 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 70 mg 0.07 0.514
2 100 mg 0.1 -
3 6 mg 0.006 0.097
4 190 mg 0.19 -
5 2 mg 0.002 -
6 24 mg 0.024 -
7 36 mg 0.036 0.149
8 1000 ml 998.207 55408.8
Solution occurs in 87 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(7.7 M, 25%)
10.0 ml 3.17 86.943
2 1.5 g 1.5 -
3 990.0 ml 988.225 54854.8
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 69 other media (first defined in medium J431 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.4 g 0.4 10.001
2 6.0 mg 0.006 -
3 8.0 mg 0.008 -
4 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 927.81 51501.3
NaHCO3 8.517 101.382
Na2HPO4 x 2H2O 0.523 -
KH2PO4 0.404 2.971
NH4Cl 0.296 5.531
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