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JCM 1097 . SEAWATER PYRUVATE THIOSULFATE MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1097 #

ml
Equipment needed: Anaerobic workbench Filter Autoclave
1 Mix components and autoclave under a N2-CO2 (4:1, v/v) atmosphere. After cooling, add the following solutions (autoclaved or *filter-sterilized):
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 20.00 g 19.608 335.521
MgCl2 x 6 H2O 3.00 g 2.941 14.467
CaCl2 x 2 H2O 0.15 g 0.147 1
NH4Cl 0.25 g 0.245 4.582
KH2PO4 0.20 g 0.196 1.441
KCl 0.50 g 0.49 6.575
RST trace elements 10.00 ml - -
Resazurin 1.00 mg 9.8e-4 0.004
Distilled water 922.50 ml - -
NaHCO3
(*, 8%) 		37.50 ml - -
RST vitamin solution
(*) 		10.00 ml - -
Sodium pyruvate
(1 M) 		20.00 ml - -
Yeast extract
(1%) 		10.00 ml - -
2 Anaerobically and aseptically distribute the medium into appropriate culture vessels (e.g., 20 ml medium in 60 ml serum bottles) under a N2-CO2 (4:1, v/v) gas stream and seal with butyl rubber stoppers. Finally, add per liter the following solution:
Reducing agent solution 10.00 ml - -

RST vitamin solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Coenzyme M 5 mg 0.005 0.035
Biotin 2 mg 0.002 0.008
Folic acid 2 mg 0.002 0.005
Pyridoxine hydrochloride 10 mg 0.01 -
Thiamine HCl 5 mg 0.005 -
Riboflavin 5 mg 0.005 0.013
Nicotinic acid 5 mg 0.005 0.041
DL-Calcium pantothenate 5 mg 0.005 0.01
p-Aminobenzoic acid 5 mg 0.005 0.036
Lipoic acid 5 mg 0.005 0.024
Distilled water 1000 ml - -

RST trace elements #

1000
Solution occurs in 1 other media (first defined in Medium J933).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 2.00 g 2 10.464
MnSO4 x n H2O 1.00 g 1 -
Fe(NH4)2(SO4)2 x 6 H2O 0.80 g 0.8 -
CoCl2 x 6 H2O 0.20 g 0.2 1.54
ZnSO4 x 7 H2O 0.20 g 0.2 0.696
CuCl2 x 2 H2O 0.02 g 0.02 0.117
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.02 g 0.02 0.083
Na2SeO4 0.02 g 0.02 0.106
Na2WO4 x 2 H2O 0.02 g 0.02 0.061
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.0 with KOH. Then proceed to add minerals.

Reducing agent solution #

1000
Solution occurs in 1 other media (first defined in Medium J521).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 12.5 g 12.5 52.044
L-Cysteine HCl x H2O 12.5 g 12.5 -
Distilled water 1000.0 ml - -
1 Autoclave and store under a N2 atmosphere.
2 Comment: For strain JCM 13588, inoculate with a 20% inoculum, and incubate the vessels in inverted position.

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 19.608 335.521
MgCl2 x 6 H2O 2.941 14.467
KCl 0.49 6.575
NH4Cl 0.245 4.582
KH2PO4 0.196 1.441
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
nutrition chemoorganotroph 1 strain
oxygen obligate anaerobe 1 strain
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