Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 3.620 | g | 3.62 | 61.944 | |
K2HPO4 | 0.260 | g | 0.26 | 1.493 | |
NH4Cl | 0.075 | g | 0.075 | 1.402 | |
Na2SO4 | 0.050 | g | 0.05 | 0.352 | |
NaNO3 | 0.850 | g | 0.85 | 10.001 | |
L-Sodium lactate | 2.240 | g | 2.24 | 19.989 | |
Yeast extract | 0.200 | g | 0.2 | - | |
Tryptone | 0.500 | g | 0.5 | 7.034 | |
FeCl2 solution | 1.000 | ml | - | - | |
Trace element solution | 1.000 | ml | - | - | |
Trace vitamins | 10.000 | ml | - | - | |
Distilled water | 935.500 | ml | - | - | |
1 Mix components thoroughly and autoclave under a N2 atmosphere. After cooling, add the following solution (filter-sterilized): | |||||
NaHCO3
(8%) |
52.500 | ml | - | - | |
2 Aseptically and anaerobically distribute the medium into culture vessels under a N2 atmosphere and add H2 at 5% volume of the gas phase. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
MgCl2 x 6 H2O | 3 | g | 3 | 14.756 |
CaCl2 x 2 H2O | 100 | mg | 0.1 | 0.68 |
ZnCl2 | 70 | mg | 0.07 | 0.514 |
MnCl2 x 4 H2O | 100 | mg | 0.1 | - |
H3BO3 | 6 | mg | 0.006 | 0.097 |
CoCl2 x 6 H2O | 190 | mg | 0.19 | 1.463 |
CuCl2 x 2 H2O | 2 | mg | 0.002 | 0.012 |
NiCl2 x 6 H2O | 24 | mg | 0.024 | 0.185 |
Na2MoO4 x 2 H2O | 36 | mg | 0.036 | 0.149 |
Distilled water | 1000 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
HCl
(7.7 M, 25%) |
10.0 | ml | 2.5 | 68.568 |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 |
Distilled water | 990.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 29.10.21 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | n.d. |
Equipment needed: | Anaerobic workbench, Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 3.62 | 61.944 |
L-Sodium lactate | 2.24 | 19.989 |
NaNO3 | 0.85 | 10.001 |
Tryptone | 0.5 | 7.034 |
K2HPO4 | 0.26 | 1.493 |
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