Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 10.0 | g | 9.891 | 169.254 | |
KCl | 0.2 | g | 0.198 | 2.654 | |
K2HPO4 | 0.2 | g | 0.198 | 1.136 | |
MgCl2 x 6 H2O | 0.1 | g | 0.099 | 0.487 | |
NH4Cl | 1.0 | g | 0.989 | 18.492 | |
Yeast extract | 0.1 | g | 0.099 | - | |
Trace vitamins | 2.0 | ml | - | - | |
Trace minerals solution | 1.0 | ml | - | - | |
Resazurin | 0.5 | mg | 4.9e-4 | 0.002 | |
Distilled water | 680.0 | ml | - | - | |
1 Mix components thoroughly and autoclave under a N2 atmosphere in a bottle capped with a butyl rubber stopper. After cooling, add the following solution to the medium: | |||||
Alkaline solution | 300.0 | ml | - | - | |
Glucose
(autoclaved, 1.0 M) |
20.0 | ml | - | - | |
2 Anaerobically and aseptically distribute the medium into culture vessels (e.g., 10 ml medium in Hungate tubes) under a N2 gas atmosphere and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solution (autoclaved and stored under a N2 gas atmosphere): | |||||
Na2S x 9 H2O
(5%) |
8.0 | ml | - | - | |
3 Adjust pH to 9.0, if necessary. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2CO3 | 2.76 | g | 9.2 | 86.802 | |
NaHCO3 | 24.00 | g | 80 | 952.302 | |
Distilled water | 300.00 | ml | - | - | |
1 Mix components thoroughly and filter-sterilize. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 1.500 | g | 1.5 | 7.848 | |
Fe(NH4)2(SO4)2 x 6 H2O | 0.200 | g | 0.2 | - | |
Na2SeO3 | 0.100 | g | 0.1 | - | |
Na2MoO4 x 2 H2O | 0.100 | g | 0.1 | 0.413 | |
Na2WO4 x 2 H2O | 1.000 | g | 1 | 3.032 | |
MnSO4 x n H2O | 0.100 | g | 0.1 | - | |
ZnSO4 x 7 H2O | 0.100 | g | 0.1 | 0.348 | |
NiCl2 x 6 H2O | 0.025 | g | 0.025 | 0.193 | |
CuSO4 x 5 H2O | 0.010 | g | 0.01 | 0.04 | |
Distilled water | 1000.000 | ml | - | - | |
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals. | |||||
2 Adjust final pH to 7.0. |
Last modified: | 22.02.22 |
Source: | JCM |
Medium type: | Complex medium |
Final pH: | 9.0 |
Equipment needed: | Anaerobic workbench, Autoclave, Filter, Hungate tubes |
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