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JCM 1103 . ANAEROLINEA MEDIUM C

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1103 #

ml
Equipment needed: Filter Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.14 g 0.137 1.005
MgCl2 x 6 H2O 0.20 g 0.195 0.961
CaCl2 x 2 H2O 0.15 g 0.146 0.996
NH4Cl 0.54 g 0.527 9.859
Yeast extract
(BD-Difco) 		2.30 g 2.246 -
Trace element solution SL-11 1.00 ml - -
Selenite-tungstate solution 0.50 ml - -
Trace vitamins 10.00 ml - -
NaHCO3 2.50 g 2.441 29.062
Resazurin 1.00 mg 9.8e-4 0.004
Distilled water 900.00 ml - -
1 Solution B:
Glucose 2.20 g 2.148 11.925
Distilled water 100.00 ml - -
2 To complete the medium, aseptically and anaerobically add 0.1 volume of Solution B to 0.9 volume of Solution A (e.g., 18 ml Solution A in 60 ml serum bottles and 2 ml Solution B). Prior to inoculation, add per liter the following solutions:
Na2S x 9 H2O
(5%) 		6.00 ml - -
L-Cysteine HCl x H2O
(5%) 		6.00 ml - -
3 Solution A:
4 Mix components except NaHCO3 and trace vitamins, and adjust pH to 6.5. Bring to a boil for 5-10 sec, then, cool down under a stream of N2-CO2 (80:20, v/v) and add NaHCO3 to the solution. Dispense Solution A into culture vessels under a stream of N2-CO2 (80:20, v/v), seal with butyl rubber stopper and autoclave. After autoclaving, stand the medium overnight. Add trace vitamins from a sterile anaerobic stock (filter-sterilized).
5 Adjust pH to 7.0 and filter-sterilize. Keep under a N2 atmosphere.

Trace element solution SL-11 #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 5.2 g 5.2 -
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 -
H3BO3 6.0 g 6 97.025
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 1000.0 ml - -
1 Adjust pH to 6.0.

Selenite-tungstate solution #

1000
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.0
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 2.441 29.062
Yeast extract 2.246 -
Glucose 2.148 11.925
NH4Cl 0.527 9.859
MgCl2 x 6 H2O 0.195 0.961
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Cultivation metadata from 3 strains BacDive

pH 6 - 8 3 strains
pH 8 - 10 3 strains
phyla 3 3 strains
nutrition chemoorganotroph 2 strains
oxygen obligate anaerobe 2 strains
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