Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2SO4 | 0.02 | g | 0.02 | 0.14 | |
KH2PO4 | 1.00 | g | 0.997 | 7.326 | |
NH4Cl | 0.10 | g | 0.1 | 1.864 | |
NaCl | 20.00 | g | 19.94 | 341.208 | |
MgCl2 x 6 H2O | 3.00 | g | 2.991 | 14.712 | |
KCl | 0.50 | g | 0.499 | 6.687 | |
CaCl2 x 2 H2O | 0.15 | g | 0.15 | 1.017 | |
FeCl2 solution | 1.00 | ml | - | - | |
Trace element solution | 1.00 | ml | - | - | |
Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
Distilled water | 950.00 | ml | - | - | |
1 Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) atmosphere. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized): | |||||
NaHCO3
(*, 8%) |
30.00 | ml | - | - | |
Vitamin solution
(*) |
1.00 | ml | - | - | |
Yeast extract
(10%) |
5.00 | ml | - | - | |
Maltose
(1 M) |
5.00 | ml | - | - | |
Na2S x 9 H2O
(5%) |
10.00 | ml | - | - | |
2 Readjust pH to 6.8 - 7.2, if necessary. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
HCl
(7.7 M, 25%) |
10.0 | ml | 2.5 | 68.568 |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 |
Distilled water | 990.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
ZnCl2 | 70 | mg | 0.07 | 0.514 |
MnCl2 x 4 H2O | 100 | mg | 0.1 | 0.505 |
H3BO3 | 6 | mg | 0.006 | 0.097 |
CoCl2 x 6 H2O | 190 | mg | 0.19 | 1.463 |
CuCl2 x 2 H2O | 2 | mg | 0.002 | 0.012 |
NiCl2 x 6 H2O | 24 | mg | 0.024 | 0.185 |
Na2MoO4 x 2 H2O | 36 | mg | 0.036 | 0.149 |
Distilled water | 1000 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Vitamin B12 | 50 | mg | 0.05 | 0.037 |
p-Aminobenzoic acid | 50 | mg | 0.05 | 0.365 |
Biotin | 10 | mg | 0.01 | 0.041 |
Nicotinic acid | 100 | mg | 0.1 | 0.812 |
DL-Calcium pantothenate | 25 | mg | 0.025 | 0.052 |
Pyridoxine hydrochloride | 250 | mg | 0.25 | - |
Thiamine HCl | 50 | mg | 0.05 | - |
Distilled water | 1000 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.0 |
Equipment needed: | Anaerobic workbench, Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 19.94 | 341.208 |
MgCl2 x 6 H2O | 2.991 | 14.712 |
KH2PO4 | 0.997 | 7.326 |
KCl | 0.499 | 6.687 |
CaCl2 x 2 H2O | 0.15 | 1.017 |
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