Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 22.00 | g | 22 | 376.454 | |
CaCl2 x 2 H2O | 0.14 | g | 0.14 | 0.952 | |
KCl | 0.34 | g | 0.34 | 4.561 | |
NH4Cl | 0.50 | g | 0.5 | 9.348 | |
MgCl2 x 6 H2O | 2.75 | g | 2.75 | 13.527 | |
K2HPO4 | 0.14 | g | 0.14 | 0.804 | |
Yeast extract
(BD-Difco) |
0.20 | g | 0.2 | - | |
Trace mineral solution | 10.00 | ml | - | - | |
Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
Distilled water | 930.00 | ml | - | - | |
1 Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) atmosphere. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized): | |||||
NaHCO3
(*, 8%) |
30.00 | ml | - | - | |
Trace vitamins
(*) |
10.00 | ml | - | - | |
Na2S x 9 H2O
(5%) |
10.00 | ml | - | - | |
L-Cysteine HCl x H2O
(5%) |
10.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Trace minerals | 1000.00 | ml | - | - |
NiCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 |
Na2SeO3 x 5 H2O | 0.30 | mg | 3.0e-4 | 0.001 |
Na2WO4 x 2 H2O | 0.40 | mg | 4.0e-4 | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 29.10.21 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | n.d. |
Equipment needed: | Anaerobic workbench, Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 22.01 | 376.625 |
MgCl2 x 6 H2O | 2.75 | 13.527 |
NH4Cl | 0.5 | 9.348 |
KCl | 0.34 | 4.561 |
Yeast extract | 0.2 | - |
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