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J1106. MARINE METHANOGENIC MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 22.00 g 22 376.438
2 0.14 g 0.14 0.952
3 0.34 g 0.34 4.561
4 0.50 g 0.5 9.347
5 2.75 g 2.75 13.527
6 0.14 g 0.14 0.804
7
(BD-Difco)
0.20 g 0.2 -
8 Trace mineral solution 10.00 ml - -
9 1.00 mg 1.0e-3 0.004
10 930.00 ml 928.333 51530.2
11
(*, 8%)
30.00 ml 5.182 61.681
12 Trace vitamins
(*)
10.00 ml - -
13
(5%)
10.00 ml - -
14
(5%)
10.00 ml - -
Instructions:
  1. Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) atmosphere. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized): #11-14
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 4 other media (first defined in medium J1084 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Trace minerals 1000.00 ml - -
2 0.03 g 0.03 -
3 0.30 mg 3.0e-4 -
4 0.40 mg 4.0e-4 -

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 948.297 52638.4
NaCl 22.01 376.609
NaHCO3 5.182 61.681
MgCl2 x 6H2O 2.75 13.527
NH4Cl 0.5 9.347
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