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JCM 1107 . LIQUID BASAL MEDIUM FOR METHANOSARCINA

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1107 #

ml
Equipment needed: Autoclave Anaerobic workbench Filter
1 Mix components thoroughly, adjust pH to 7.2 and autoclave under a N2-CO2 (80:20, v/v) gas atmosphere. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.50 g 0.49 3.599
NH4Cl 0.40 g 0.392 7.324
MgCl2 x 6 H2O 0.40 g 0.392 1.927
CaCl2 x 2 H2O 0.05 g 0.049 0.333
NaCl 0.40 g 0.392 6.704
MOPS 4.20 g 4.114 19.657
Trace elements solution 1.00 ml - -
Resazurin 1.00 mg 9.8e-4 0.004
Distilled water 930.00 ml - -
NaHCO3
(*, 8%) 		50.00 ml - -
Vitamin solution
(*) 		10.00 ml - -
Methanol
(v/v, 50%) 		10.00 ml - -
2 Aseptically distribute the medium into culture vessels under the same gas mixture and seal with butyl rubber stoppers. Finally, add per liter the following solutions from anaerobic stocks (autoclaved and stored under a N2 atmosphere):
L-Cysteine HCl x H2O
(5%) 		10.00 ml - -
Na2S x 9 H2O
(5%) 		10.00 ml - -
3 Readjust pH to 7.2, if necessary.

Trace elements solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid trisodium salt monohydrate 11.10 g 11.1 40.349
FeSO4 x 7 H2O 5.55 g 5.55 19.963
Na2SeO3 x 5 H2O 0.30 g 0.3 1.141
CoCl2 x 6 H2O 1.00 g 1 7.702
MnSO4 x 5 H2O 1.00 g 1 4.148
Na2MoO4 x 2 H2O 1.00 g 1 4.133
Na2WO4 x 2 H2O 1.00 g 1 3.032
ZnSO4 x 7 H2O 1.00 g 1 3.478
NiCl2 x 6 H2O 3.00 g 3 23.148
H3BO3 0.10 g 0.1 1.617
CuSO4 x 5 H2O 0.10 g 0.1 0.401
Distilled water 1000.00 ml - -

Vitamin solution #

1000
Solution occurs in 2 other media (first defined in Medium J944).
Compound Amount Unit Conc. [g/L] Conc. [mM]
p-Aminobenzoic acid 10 mg 0.01 0.073
Nicotinic acid 10 mg 0.01 0.081
DL-Calcium pantothenate 10 mg 0.01 0.021
Pyridoxine hydrochloride 10 mg 0.01 -
Riboflavin 10 mg 0.01 0.027
Thiamine HCl 10 mg 0.01 -
Biotin 5 mg 0.005 0.02
Folic acid 5 mg 0.005 0.011
Lipoic acid 5 mg 0.005 0.024
Vitamin B12 5 mg 0.005 0.004
Distilled water 1000 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Archaea
Medium type: Defined medium
Final pH: 7.2
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MOPS 4.114 19.657
KH2PO4 0.49 3.599
NH4Cl 0.392 7.324
NaCl 0.392 6.704
MgCl2 x 6 H2O 0.392 1.927
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Cultivation metadata from 1 strains BacDive

halophily non-halophilic 1 strain
pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
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