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JCM 1108 . TEPIDIBACILLUS B5 MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1108 #

ml
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 1.00 g 0.997 17.06
KCl 0.50 g 0.499 6.687
MgCl2 x 6 H2O 0.40 g 0.399 1.962
CaCl2 x 2 H2O 0.10 g 0.1 0.678
KH2PO4 0.20 g 0.199 1.465
NH4Cl 0.50 g 0.499 9.32
NaNO3 0.42 g 0.419 4.927
Yeast extract 1.00 g 0.997 -
Trace element solution 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Distilled water 980.00 ml - -
1 Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) gas atmosphere. After cooling, add the following solutions (autoclaved or *filter-sterilized):
Vitamin solution
(*) 		1.00 ml - -
Glucose
(1 M) 		5.00 ml - -
NaHCO3
(*, 8%) 		15.00 ml - -
2 Adjust pH to 7.0, if necessary.

Trace element solution #

Solution occurs in 5 other media (first defined in Medium J301).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 12.80 g - -
FeSO4 x 7 H2O 0.42 g - -
H3BO3 0.01 g - -
MnCl2 x 4 H2O 0.10 g - -
CoCl2 x 6 H2O 0.17 g - -
CuCl2 x 2 H2O 0.02 g - -
ZnSO4 x 7 H2O 0.21 g - -
Na2MoO4 x 2 H2O 0.01 g - -
1 Dissolve nitrilotriacetic acid in 800 ml distilled water and adjust pH to 6.5 with NaOH. Then proceed to add the minerals and bring volume to 1.0 L.

Selenite-tungstate solution #

1000
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -

Vitamin solution #

100
Solution occurs in 14 other media (first defined in Medium J403).
Compound Amount Unit Conc. [g/L] Conc. [mM]
p-Aminobenzoic acid 4 mg 0.04 0.292
Biotin 1 mg 0.01 0.041
Nicotinic acid 10 mg 0.1 0.812
DL-Calcium pantothenate 5 mg 0.05 0.105
Pyridoxine hydrochloride 10 mg 0.1 -
Sodium phosphate buffer
(10 mM, pH 7.1) 		100 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 0.997 17.06
Yeast extract 0.997 -
NH4Cl 0.499 9.32
KCl 0.499 6.687
NaNO3 0.419 4.927
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