J1111
1111 .
ANAEROBIC ALKALINE LB MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1111 #
ml
Equipment needed: Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| LB broth powder | 25 | g | 24.802 | - | |
| Tris base | 3 | g | 2.976 | 24.568 | |
| Distilled water | 800 | ml | - | - | |
| 1 Mix components thoroughly, adjust pH to 9.5 with NaOH and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add aseptically and anaerobically the following solutions (autoclaved and stored under N2): | |||||
|
Na2CO3
(8%) |
200 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
8 | ml | - | - | |
Metadata
| Last modified: | 24.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 9.5 |
| Equipment needed: | Anaerobic workbench, Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| LB broth powder | 24.802 | - |
| Tris base | 2.976 | 24.568 |
| Distilled water | - | - |
| Na2CO3 | - | - |
| Na2S x 9 H2O | - | - |
