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JCM 1112 . MINERAL MEDIUM WITH GLUCOSE

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1112 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 2.5 g 2.444 41.817
KCl 0.3 g 0.293 3.934
K2HPO4 0.3 g 0.293 1.684
MgSO4 x 7 H2O 0.1 g 0.098 0.397
NH4Cl 1.0 g 0.978 18.275
CaSO4 x 7 H2O 12.5 mg 0.012 0.09
Yeast extract 0.1 g 0.098 -
Trace vitamins 2.0 ml - -
Trace minerals solution 1.0 ml - -
Distilled water 1000.0 ml - -
1 Mix components thoroughly, adjust pH to 7.4 and autoclave. After cooling, add the following solution (autoclaved):
Glucose
(1 M) 		20.0 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Trace minerals solution #

1000
Solution occurs in 5 other media (first defined in Medium J228).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.500 g 1.5 7.848
Fe(NH4)2(SO4)2 x 6 H2O 0.200 g 0.2 -
Na2SeO3 0.100 g 0.1 -
Na2MoO4 x 2 H2O 0.100 g 0.1 0.413
Na2WO4 x 2 H2O 1.000 g 1 3.032
MnSO4 x n H2O 0.100 g 0.1 -
ZnSO4 x 7 H2O 0.100 g 0.1 0.348
NiCl2 x 6 H2O 0.025 g 0.025 0.193
CuSO4 x 5 H2O 0.010 g 0.01 0.04
Distilled water 1000.000 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals.
2 Adjust final pH to 7.0.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.4
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 2.444 41.817
NH4Cl 0.978 18.275
KCl 0.293 3.934
K2HPO4 0.293 1.684
MgSO4 x 7 H2O 0.098 0.397
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Cultivation metadata from 2 strains BacDive

pH 6 - 8 2 strains
pH 8 - 10 2 strains
phyla 1 2 strains
nutrition chemoorganoheterotroph 1 strain
oxygen facultative anaerobe 2 strains
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