Name: VARUNAVIBRIO MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J1115 #

Equipment needed: Autoclave Filter

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NaCl	15.00	g	14.837	253.881
K₂HPO₄	0.14	g	0.138	0.795
CaCl₂ x 2 H₂O	0.14	g	0.138	0.942
MgSO₄ x 7 H₂O	3.40	g	3.363	13.644
MgCl₂ x 6 H₂O	4.18	g	4.135	20.337
KCl	0.33	g	0.326	4.378
NiCl₂ x 6 H₂O	0.50	mg	4.9e-4	0.004
Na₂SeO₃ x 5 H₂O	0.50	mg	4.9e-4	0.002
Fe(NH₄)₂(SO₄)₂ x 6 H₂O	0.01	g	0.01	-
Trace minerals	10.00	ml	-	-
NH₄Cl	0.25	g	0.247	4.623
Distilled water	950.00	ml	-	-
1 Mix components thoroughly and autoclave. After cooling, add the following solutions (autoclaved or *filter-sterilized):
NaHCO₃ (*, 8%)	15.00	ml	-	-
Na₂S₂O₃ x 5 H₂O (*, 10%)	15.00	ml	-	-
KNO₃ (10%)	20.00	ml	-	-
Trace vitamins (see Medium No. 197) (*)	1.00	ml	-	-
2 Distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) under a stream of N₂-CO₂ (4:1, v/v) and seal with butyl rubber stoppers. Pressurize the vessels to 200 kPa with the same gas mixture.

Trace minerals #

1000

Solution occurs in 97 other media (first defined in Medium J151).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Nitrilotriacetic acid	1.50	g	1.5	7.848
MgSO₄ x 7 H₂O	3.00	g	3	12.172
MnSO₄ x n H₂O	0.50	g	0.5	-
NaCl	1.00	g	1	17.112
FeSO₄ x 7 H₂O	0.10	g	0.1	0.36
CoSO₄ x 7 H₂O	0.10	g	0.1	0.645
CaCl₂ x 2 H₂O	0.10	g	0.1	0.68
ZnSO₄ x 7 H₂O	0.10	g	0.1	0.348
CuSO₄ x 5 H₂O	0.01	g	0.01	0.04
AlK(SO₄)₂	0.01	g	0.01	0.039
H₃BO₃	0.01	g	0.01	0.162
Na₂MoO₄ x 2 H₂O	0.01	g	0.01	0.041
Distilled water	1000.00	ml	-	-
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

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