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JCM 1116 . IRD MARINE DESULFOVIBRIO MEDIUM-2

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.30 g 0.303 5.671
KH2PO4 0.20 g 0.202 1.486
NaCl 30.00 g 30.334 519.057
KCl 0.50 g 0.506 6.782
CaCl2 x 2 H2O 0.15 g 0.152 1.032
MgCl2 x 6 H2O 0.40 g 0.404 1.989
Na2SO4 2.80 g 2.831 19.932
Yeast extract 0.50 g 0.506 -
Trace element solution 1.00 ml - -
L-Cysteine HCl x H2O 0.50 g 0.506 -
Resazurin 1.00 mg 0.001 0.004
Distilled water 920.00 ml - -
1 Mix components and adjust pH to 7.0. Bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas stream. Distribute the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized):
Sodium lactate
(1 M)
20.00 ml - -
NaHCO3
(*, 5%)
40.00 ml - -
2 Prior to use, add per liter the following solution to reduce the medium (autoclaved and stored under N2):
Na2S x 9 H2O
(5%)
8.00 ml - -
Solution occurs in 38 other media (first defined in Medium J439).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
12.5 ml - -
FeSO4 x 7 H2O 2.1 g 2.1 7.554
H3BO3 30.0 mg 0.03 0.485
MnCl2 x 4 H2O 100.0 mg 0.1 -
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
ZnSO4 x 7 H2O 144.0 mg 0.144 0.501
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 987.0 ml - -

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Anaerobic workbench, Autoclave, Filter

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 30.334 519.057
Na2SO4 2.831 19.932
KCl 0.506 6.782
L-Cysteine HCl x H2O 0.506 -
Yeast extract 0.506 -
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Cultivation metadata from 1 strains BacDive

halophily moderately halophilic 1 strain
pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
nutrition heterotroph 1 strain
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