Name: IRD MARINE DESULFOVIBRIO MEDIUM-2
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J1116 #

Equipment needed: Filter Autoclave Anaerobic workbench

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NH₄Cl	0.30	g	0.303	5.671
KH₂PO₄	0.20	g	0.202	1.486
NaCl	30.00	g	30.334	519.057
KCl	0.50	g	0.506	6.782
CaCl₂ x 2 H₂O	0.15	g	0.152	1.032
MgCl₂ x 6 H₂O	0.40	g	0.404	1.989
Na₂SO₄	2.80	g	2.831	19.932
Yeast extract	0.50	g	0.506	-
Trace element solution	1.00	ml	-	-
L-Cysteine HCl x H₂O	0.50	g	0.506	-
Resazurin	1.00	mg	0.001	0.004
Distilled water	920.00	ml	-	-
1 Mix components and adjust pH to 7.0. Bring to a boil and cool down under a N₂-CO₂ (4:1, v/v) gas stream. Distribute the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized):
Sodium lactate (1 M)	20.00	ml	-	-
NaHCO₃ (*, 5%)	40.00	ml	-	-
2 Prior to use, add per liter the following solution to reduce the medium (autoclaved and stored under N₂):
Na₂S x 9 H₂O (5%)	8.00	ml	-	-

Trace element solution #

1000

Solution occurs in 38 other media (first defined in Medium J439).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
HCl (25%)	12.5	ml	-	-
FeSO₄ x 7 H₂O	2.1	g	2.1	7.554
H₃BO₃	30.0	mg	0.03	0.485
MnCl₂ x 4 H₂O	100.0	mg	0.1	-
CoCl₂ x 6 H₂O	190.0	mg	0.19	1.463
NiCl₂ x 6 H₂O	24.0	mg	0.024	0.185
CuCl₂ x 2 H₂O	2.0	mg	0.002	0.012
ZnSO₄ x 7 H₂O	144.0	mg	0.144	0.501
Na₂MoO₄ x 2 H₂O	36.0	mg	0.036	0.149
Distilled water	987.0	ml	-	-

Last modified:	22.02.22
Source:	JCM
Taxonomic range:	Bacteria
Medium type:	Complex medium
Final pH:	7.0
Equipment needed:	Anaerobic workbench, Autoclave, Filter

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