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JCM 1121 . AZC 1502 MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1121 #

ml
Equipment needed: Autoclave Hungate tubes Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.10 g 0.096 0.705
NH4Cl 0.10 g 0.096 1.792
KCl 0.05 g 0.048 0.643
CaCl2 x 2 H2O 0.05 g 0.048 0.326
MgCl2 x 6 H2O 0.10 g 0.096 0.472
Na2S2O3 x 5 H2O 3.20 g 3.068 12.362
FeCl2 solution 1.00 ml - -
Trace element solution 1.00 ml - -
Fe-Ni-WO-Se solution 1.00 ml - -
Distilled water 1000.00 ml - -
1 Mix components thoroughly and adjust pH to 5.0. Bring to a boil and cool under a N2 gas stream. Distribute the medium into culture vessels (e.g., 5 ml of the medium in the Hungate tubes) under the same gas atmosphere, seal with butyl rubber stoppers, and autoclave. After cooling, replace the gas phase with a H2-CO2 (4:1, v/v) gas mixture and add per liter the following solution (freshly prepared and filter-sterilized):
Sodium acetate
(1 M) 		10.00 ml - -
Yeast extract
(1%) 		10.00 ml - -
Sodium dithionite
(0.1%) 		20.00 ml - -
2 Pressurize the inoculated culture vessels to 100 kPa H2-CO2 (4:1, v/v).

Fe-Ni-WO-Se solution #

1000
Solution occurs in 3 other media (first defined in Medium J1121).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 1.42 g 1.42 5.108
NiSO4 x 7 H2O 1.60 g 1.6 10.339
Na2WO4 x 2 H2O 38.00 mg 0.038 0.115
Na2SeO3 x 5 H2O 3.00 mg 0.003 0.011
Distilled water 1000.00 ml - -

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Medium type: Complex medium
Final pH: 5.0
Equipment needed: Autoclave, Filter, Hungate tubes
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2S2O3 x 5 H2O 3.068 12.362
NH4Cl 0.096 1.792
KH2PO4 0.096 0.705
MgCl2 x 6 H2O 0.096 0.472
KCl 0.048 0.643
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