Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 0.20 | g | 0.189 | 1.385 | |
NH4Cl | 0.25 | g | 0.236 | 4.405 | |
KCl | 0.72 | g | 0.679 | 9.103 | |
NaCl | 26.00 | g | 24.505 | 419.322 | |
Na2SO4 | 4.00 | g | 3.77 | 26.542 | |
FeCl2 solution | 1.00 | ml | - | - | |
Trace element solution | 1.00 | ml | - | - | |
Selenite-tungstate solution | 1.00 | ml | - | - | |
Resazurin | 1.00 | mg | 9.4e-4 | 0.004 | |
Distilled water | 960.00 | ml | - | - | |
1 Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas atmosphere. After cooling, add the following solutions (autoclaved or *filter-sterilized): | |||||
NaHCO3
(*, 8%) |
30.00 | ml | - | - | |
MgCl2 x 6 H2O
(1 M) |
40.00 | ml | - | - | |
CaCl2 x 2 H2O
(1 M) |
10.00 | ml | - | - | |
Vitamin solution (see Medium No. 403)
(*) |
1.00 | ml | - | - | |
Thiamine solution (see Medium No. 403)
(*) |
1.00 | ml | - | - | |
Vitamin B12 solution (see Medium No. 403)
(*) |
1.00 | ml | - | - | |
Glucose
(*, 1 M) |
10.00 | ml | - | - | |
2 Aseptically and anaerobically, distribute the medium into culture vessels under the same gas mixture (e.g., 20 ml in 60 ml-serum bottles) and seal with butyl rubber stoppers. Prior to use, add per liter the following solution to reduce the medium (autoclaved and stored under N2): | |||||
Na2S x 9 H2O
(5%) |
5.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
HCl
(7.7 M, 25%) |
10.0 | ml | 2.5 | 68.568 |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 |
Distilled water | 990.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
ZnCl2 | 70 | mg | 0.07 | 0.514 |
MnCl2 x 4 H2O | 100 | mg | 0.1 | 0.505 |
H3BO3 | 6 | mg | 0.006 | 0.097 |
CoCl2 x 6 H2O | 190 | mg | 0.19 | 1.463 |
CuCl2 x 2 H2O | 2 | mg | 0.002 | 0.012 |
NiCl2 x 6 H2O | 24 | mg | 0.024 | 0.185 |
Na2MoO4 x 2 H2O | 36 | mg | 0.036 | 0.149 |
Distilled water | 1000 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaOH | 0.4 | g | 0.4 | 10.001 |
Na2SeO3 x 5 H2O | 6.0 | mg | 0.006 | 0.023 |
Na2WO4 x 2 H2O | 8.0 | mg | 0.008 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 29.10.21 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | n.d. |
Equipment needed: | Anaerobic workbench, Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 24.505 | 419.322 |
Na2SO4 | 3.77 | 26.542 |
KCl | 0.679 | 9.103 |
NH4Cl | 0.236 | 4.405 |
KH2PO4 | 0.189 | 1.385 |
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