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JCM 1125 . ARTFICIAL FRESHWATER MEDIUM II

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1125 #

ml
Equipment needed: Autoclave Anaerobic workbench Filter
1 Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas atmosphere. After cooling, add the following solutions (autoclaved or *filter-sterilized):
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.20 g 0.197 1.447
NH4Cl 0.25 g 0.246 4.6
KCl 0.30 g 0.295 3.961
Na2SO4 1.40 g 1.378 9.701
FeCl2 solution 1.00 ml - -
Trace element solution 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Resazurin 1.00 mg 9.8e-4 0.004
Distilled water 960.00 ml - -
NaHCO3
(*, 8%) 		30.00 ml - -
MgCl2 x 6 H2O
(1 M) 		2.50 ml - -
CaCl2 x 2 H2O
(1 M) 		1.00 ml - -
Vitamin solution (see Medium No. 403)
(*) 		1.00 ml - -
Thiamine solution (see Medium No. 403)
(*) 		1.00 ml - -
Vitamin B12 solution (see Medium No. 403)
(*) 		1.00 ml - -
Yeast extract
(10%) 		1.00 ml - -
glycerin
(*, 1 M) 		10.00 ml - -
2 Aseptically and anaerobically, distribute the medium into culture vessels under the same gas mixture (e.g., 20 ml in 60 ml-serum bottles) and seal with butyl rubber stoppers. Prior to use, add per liter the following solution to reduce the medium (autoclaved and stored under N2):
Na2S x 9 H2O
(5%) 		5.00 ml - -
3 Comment:
4 For cultivation of JCM 31104, use 1 M sodium lactate solution instead of 1 M glycerin solution (final 10 mM).

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Selenite-tungstate solution #

1000
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2SO4 1.378 9.701
KCl 0.295 3.961
NH4Cl 0.246 4.6
KH2PO4 0.197 1.447
FeCl2 x 4 H2O 0.001 0.007
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Cultivation metadata from 3 strains BacDive

pH 6 - 8 2 strains
pH 8 - 10 1 strain
phyla 1 3 strains
oxygen anaerobe 3 strains
temperature mesophilic 2 strains
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