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JCM 1130 . YCFA MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1130 #

ml
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Tryptone
(BD-Difco) 		10.0 g 10.235 143.999
Yeast extract
(BD-Difco) 		2.5 g 2.559 -
NaHCO3 4.0 g 4.094 48.736
Glucose 2.0 g 2.047 11.363
Maltose 2.0 g 2.047 5.98
Cellobiose 2.0 g 2.047 5.98
L-Cysteine HCl x H2O 1.0 g 1.024 -
Mineral solution I 150.0 ml - -
Mineral solution II 150.0 ml - -
Resazurin 1.0 mg 0.001 0.004
VFA mix 6.2 ml - -
Hemin solution 10.0 ml - -
Vitamin solution I 1.0 ml - -
Distilled water 660.0 ml - -
1 Adjust pH to 7.45.
2 After autoclaving, add filter-sterilized 1.0 ml Vitamin solution II (see below).

Mineral solution I #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 3 g 3 17.224
Distilled water 1000 ml - -

Mineral solution II #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 3.0 g 3 22.045
(NH4)2SO4 6.0 g 6 45.406
NaCl 6.0 g 6 102.669
MgSO4 x 7 H2O 0.6 g 0.6 2.434
CaCl2 x 2 H2O 0.6 g 0.6 4.081
Distilled water 1000.0 ml - -

VFA mix #

26
Compound Amount Unit Conc. [g/L] Conc. [mM]
Acetic acid 17 ml - -
Propionic acid 6 ml - -
n-Valeric acid 1 ml - -
iso-Valeric acid 1 ml - -
iso-Butyric acid 1 ml - -

Hemin solution #

25
Compound Amount Unit Conc. [g/L] Conc. [mM]
KOH 0.28 g 11.2 199.622
Ethanol 25.00 ml - -
Hemin 0.10 g 4 6.136
1 Adjust volume to 100 ml with distilled water.

Vitamin solution I #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 5 mg 0.005 0.02
Vitamin B12 5 mg 0.005 0.004
p-Aminobenzoic acid 15 mg 0.015 0.109
Folic acid 25 mg 0.025 0.057
Pyridoxine hydrochloride 75 mg 0.075 -
Distilled water 500 ml - -
1 Vitamin solution II:
Thiamine HCl 25 mg 0.025 -
Riboflavin 25 mg 0.025 0.066
Distilled water 500 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.4
Equipment needed: Filter
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Associated strains

All strains for this medium: 66

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Tryptone 10.235 143.999
NaHCO3 4.094 48.736
Yeast extract 2.559 -
Glucose 2.047 11.363
Cellobiose 2.047 5.98
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Cultivation metadata from 66 strains BacDive

pH 6 - 8 4 strains
pH 4 - 6 3 strains
phyla 4 67 strains
oxygen anaerobe 38 strains
oxygen obligate anaerobe 4 strains
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